机构：[1]Department of Urology Surgery, Daqing Oilfield General Hospital, Daqing, China[2]Department of Nephrology, the First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan Province, China内科科室肾脏内科昆明医科大学附属第一医院[3]Yunnan Key Laboratory of Laboratory Medicine, the First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan Province, China昆明医科大学附属第一医院
OBJECTIVE: To uncover the role of miRNA-1246 in influencing the proliferative and migratory capacities of RCC by binding CX-CR4 and downregulating its level. PATIENTS AND METHODS: Relative levels of miRNA-1246 and CXCR4 in 40 paired RCC tissues and adjacent normal tissues were determined. The binding relationship between miRNA-1246 and CXCR4 was confirmed by Dual-Lu-ciferase reporter assay. Proliferative and migratory abilities of RCC regulated by miRNA-1246 and CXCR4 were assessed. RESULTS: MiRNA-1246 was downregulated in RCC tissues and cell lines. Overexpression of miRNA-1246 attenuated proliferative and migratory capacities of 786-O and 769-P cells. CXCR4 was the direct target of miRNA-1246 and its level was negatively regulated by miRNA-1246. Silence of CXCR4 inhibited RCC to proliferate and migrate. CONCLUSIONS: MiRNA-1246 attenuates proliferative and migratory abilities of RCC by downregulating CXCR4. MiRNA-1246/CXCR4 axis could be potential therapeutic target for RCC.
基金：
Yunnan Applied Basic Research Projects -Union Foundation [2018FE001-032]; Yunnan Basic Research Projects General Program [2019FB092]
第一作者机构：[1]Department of Urology Surgery, Daqing Oilfield General Hospital, Daqing, China
通讯作者：
通讯机构：[2]Department of Nephrology, the First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan Province, China[3]Yunnan Key Laboratory of Laboratory Medicine, the First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan Province, China
推荐引用方式(GB/T 7714)：
Liu H-T,Fan W-X.MiRNA-1246 suppresses the proliferation and migration of renal cell carcinoma through targeting CXCR4[J].EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES.2020,24(11):5979-5987.doi:10.26355/eurrev_202006_21491.
APA：
Liu, H-T&Fan, W-X.(2020).MiRNA-1246 suppresses the proliferation and migration of renal cell carcinoma through targeting CXCR4.EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES,24,(11)
MLA：
Liu, H-T,et al."MiRNA-1246 suppresses the proliferation and migration of renal cell carcinoma through targeting CXCR4".EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES 24..11(2020):5979-5987
