机构：[1]Family Planning Research Institute, Tongji Medical College, Huazhong University of Science and Technology, No. 13 Hang-kong Road, Wuhan 430030, China.[2]Department of Obstetrics and Gynecological Ultrasound Imaging, Renmin Hospital of Wuhan University, No. 99 Zhang zhidong Road, No. 238 Jiefang Road, Wuhan 430060, China.[3]Department of Reproduction and Genetics, The First Affiliated Hospital of Kunming Medical University, No. 295 Xichang Road, Kunming 650032, China.外科科室医技科室生殖遗传科医学影像中心CT室昆明医科大学附属第一医院[4]Reproductive Medicine Center, Tongji Medical College, Huazhong University of Science and Technology, No. 128 Sanyang Road, Wuhan 430010, China.
Cold-inducible RNA-binding protein (CIRBP) is reduced by scrotal hyperthermia in cryptorchidism, varicocoele and heat treatment, but there is no direct evidence clarifying the relationship between CIRBP and spermatogenesis. The aim of this study was to investigate the expression of CIRBP in GC2-spd cells (a mouse spermatocyte cell line) before and after heat treatment, and to determine the effects of the downregulation or overexpression of CIRBP on spermatocyte cell proliferation and apoptosis. GC2-spd cells overexpressing CIRBP and GC2-spd cells in CIRBP was knocked down were constructed to investigate the function of CIRBP in cell proliferation and apoptosis using a cell counting kit-8 and flow cytometry respectively. In addition, proliferation and apoptosis were evaluated in GC2-spd cells that had been heated for 30 or 60min, and were analysed 12, 24, and 48h after heat treatment. Heat treatment clearly suppressed the proliferation of GC2-spd cells, and upregulation of CIRBP expression in GC2-spd cells promoted cell proliferation and decreased apoptosis before and after heat stress; in contrast, downregulation of CIRBP expression inhibited cell proliferation and increased apoptosis. These findings suggest that CIRBP exerts a protective effect against spermatogenic injury caused by heat stress.
基金：
This study was supported by the National Natural Science Foundation of
China (Grant no. 81771570, 81671507) and the National Key Research and
Development Program of China (No. 2016YFC1000903).
第一作者机构：[1]Family Planning Research Institute, Tongji Medical College, Huazhong University of Science and Technology, No. 13 Hang-kong Road, Wuhan 430030, China.
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
Guiping Cheng,Dandan Ke,Meng Rao,et al.Effects of cold-inducible RNA-binding protein on the proliferation and apoptosis of spermatogenic cells invitro following heat stress[J].REPRODUCTION FERTILITY AND DEVELOPMENT.2019,31(5):953-961.doi:10.1071/RD18469.
APA：
Guiping Cheng,Dandan Ke,Meng Rao,Shifu Hu,Yingying Wang...&Wei Xia.(2019).Effects of cold-inducible RNA-binding protein on the proliferation and apoptosis of spermatogenic cells invitro following heat stress.REPRODUCTION FERTILITY AND DEVELOPMENT,31,(5)
MLA：
Guiping Cheng,et al."Effects of cold-inducible RNA-binding protein on the proliferation and apoptosis of spermatogenic cells invitro following heat stress".REPRODUCTION FERTILITY AND DEVELOPMENT 31..5(2019):953-961
