机构：[a]Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China内科系统神经内科(ICU)重庆医科大学附属第一医院[b]Chongqing Key Laboratory of Neurobiology, Chongqing 400016, China[c]Institute of Neuroscience and the Collaborative Innovation Center for Brain Science, Chongqing Medical University, Chongqing 400016, China[d]Department of Clinical Laboratory, The First Affiliated Hospital of Kunming Medical University, Kunming 650032, China昆明医科大学附属第一医院医学检验科医技科室[e]School of Public Health and Management, Chongqing Medical University, Chongqing 400016, China[f]Department of Laboratory Animal Science, College of Basic Medical Sciences, Third Military Medical University, 400038 Chongqing, China
The gut microbiome is composed of an enormous number of microorganisms, generally regarded as commensal bacteria. Resident gut bacteria are an important contributor to health and significant evidence suggests that the presence of healthy and diverse gut microbiota is important for normal cognitive and emotional processing. Here we measured the expression of monoamine neurotransmitter-related genes in the hippocampus of germ-free (GF) mice and specific-pathogen-free (SPF) mice to explore the effect of gut microbiota on hippocampal monoamine functioning. In total, 19 differential expressed genes (Htr7, Htr1f, Htr3b, Drd3, Ddc, Maob, Tdo2, Fos, Creb1, Akt1, Gsk3a, Pik3ca, Pla2g5, Cyp2d22, Grk6, Ephbl, Slc18a1, Nr4a1, Gdnf) that could discriminate between the two groups were identified. Interestingly, GF mice displayed anxiolytic-like behavior compared to SPF mice, which were not reversed by colonization with gut microbiota from SPF mice. Besides, colonization of adolescent GF mice by gut microbiota was not sufficient to reverse the altered gene expression associated with their GF status. Taking these findings together, the absence of commensal microbiota during early life markedly affects hippocampal monoamine gene-regulation, which was associated with anxiolytic behaviors and monoamine neurological signs.
基金：
This work was supported by the National Key R&D Program of
China (Grant No. 2016YFC1307200 and 2017YFA0505700), the
National Natural Science Foundation of China (Grant No. 81771490
and 81371310).
第一作者机构：[a]Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China[b]Chongqing Key Laboratory of Neurobiology, Chongqing 400016, China[c]Institute of Neuroscience and the Collaborative Innovation Center for Brain Science, Chongqing Medical University, Chongqing 400016, China[d]Department of Clinical Laboratory, The First Affiliated Hospital of Kunming Medical University, Kunming 650032, China
共同第一作者：
通讯作者：
通讯机构：[*1]Department of Laboratory Animal Science, College of Basic Medical Sciences, Third Military Medical University, Gaotanyan Street, Chongqing 400038, China.[*2]Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, 1 Youyi Road, Yuzhong District, 400016 Chongqing, China.
推荐引用方式(GB/T 7714)：
Jun-Xi Pan,Feng-Li Deng,Ben-Hua Zeng,et al.Absence of gut microbiota during early life affects anxiolytic Behaviors and monoamine neurotransmitters system in the hippocampal of mice[J].JOURNAL OF THE NEUROLOGICAL SCIENCES.2019,400:160-168.doi:10.1016/j.jns.2019.03.027.
APA：
Jun-Xi Pan,Feng-Li Deng,Ben-Hua Zeng,Peng Zheng,Wei-Wei Liang...&Peng Xie.(2019).Absence of gut microbiota during early life affects anxiolytic Behaviors and monoamine neurotransmitters system in the hippocampal of mice.JOURNAL OF THE NEUROLOGICAL SCIENCES,400,
MLA：
Jun-Xi Pan,et al."Absence of gut microbiota during early life affects anxiolytic Behaviors and monoamine neurotransmitters system in the hippocampal of mice".JOURNAL OF THE NEUROLOGICAL SCIENCES 400.(2019):160-168
