机构：[1]Department of Gastroenterology, First Affiliated Hospital of Kunming Medical University, Yunnan Institute of Digestive Diseases, Kunming, China内科科室外科科室消化内科普通外科普通外一科(胃肠与疝外科)昆明医科大学附属第一医院[2]Department of Gastroenterology, The Second People’s Hospital of Yunnan Province, Kunming,China[3]Department of Medical Oncology, The Second People’s Hospital of Yunnan Province, Kunming,China[4]Department of Gastrointestinal Surgery, First Affiliated Hospital of Kunming Medical University,Kunming, China外科科室普通外科普通外一科(胃肠与疝外科)昆明医科大学附属第一医院
OBJECTIVE: The aim of this study was to investigate the effects of micro-ribonucleic acid (miR)-101 on the proliferation and apoptosis of gastric mucosal epithelial cells through nuclear factor erythroid 2-related factor 2 (Nrf2)/antioxidant response element (ARE) signaling pathway. MATERIALS AND METHODS: Human gastric epithelial AGS (CRL-1739) cells were cultured in vitro. MiR-101 down-regulation or over-expression was achieved by transfection of inhibitors, or miRNA mimics, respectively. The apoptosis rate was detected by flow cytometry. Meanwhile, the targets of miR-101 were verified by the Dual-Luciferase reporter gene assay. Furthermore, the changes in protein levels were measured via Western blotting (WB). RESULTS: Up-regulation of miR-101 significantly promoted the apoptosis of gastric mucosal epithelial cells. The 3'-untranslated region (3'-UTR) of Nrf2 was highly conserved to combine with miR-101. The Luciferase reporter gene assay showed that transfection of miR-101 mimics could remarkably inhibit the relative Luciferase activity in cells. In addition, miR-101 overexpression markedly down-regulated the messenger RNA (mRNA) and protein expressions of Nrf2 in cells. CONCLUSIONS: MiR-101 plays an important role in regulating the proliferation and apoptosis of gastric mucosal epithelial cells by targeting the Nrf2-ARE signaling pathway.
基金：
Leader Training Program in Medical Subjects of Health and Family Planning commission of Yunnan Province [D-201657]; Scientific Research Fund General Projects of Yunnan Provincial Department of Education [2015Y150]
第一作者机构：[1]Department of Gastroenterology, First Affiliated Hospital of Kunming Medical University, Yunnan Institute of Digestive Diseases, Kunming, China
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通讯作者：
推荐引用方式(GB/T 7714)：
Dong X-Q,Zhang Y-H,Shang X-Q,et al.Effects of miR-101 on the proliferation and apoptosis of gastric mucosal epithelial cells via Nrf2/ARE signaling pathway[J].EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES.2019,23(12):5187-5194.doi:10.26355/eurrev_201906_18183.
APA：
Dong, X-Q,Zhang, Y-H,Shang, X-Q&Zeng, Y-J.(2019).Effects of miR-101 on the proliferation and apoptosis of gastric mucosal epithelial cells via Nrf2/ARE signaling pathway.EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES,23,(12)
MLA：
Dong, X-Q,et al."Effects of miR-101 on the proliferation and apoptosis of gastric mucosal epithelial cells via Nrf2/ARE signaling pathway".EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES 23..12(2019):5187-5194
