Background Lactic acid sting test (LAST) is a classical method to identify sensitive skin. However, some subjects with self-perceived sensitive skin are negative for LAST. Objective To determine whether LAST scores are associated with specific phenotype of sensitive skin. Methods A total of 292 subjects with self-perceived sensitive skin were enrolled in this study. The Sensitive Scale was used to evaluate the severity of burning, stinging, itching, tautness, erythema and scaling based on 0-10 scale scores. In addition to the assessment of LAST scores, epidermal biophysical properties were measured using an MPA system. Results The Sensitive Scale scores of stinging, itching, tautness and scaling were significantly different between the LAST-positive and -negative groups. However, burning and erythema scores did not differ between the LAST-positive and -negative groups. LAST scores were positively correlated with the Sensitive Scale scores for stinging, itching, tautness and scaling, but not for burning and erythema scores. Moreover, LAST scores negatively correlated with stratum corneum hydration, but positively with transepidermal water loss (TEWL) rates. Conclusions Lactic acid sting test scores positively correlated with TEWL rates. LAST scores could be used to identify subjects with sensitive skin characterized mainly by stinging and itching, but not those mainly by burning and erythema.