机构：[1]Department of Orthopaedics, The First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan, China外科科室骨科昆明医科大学附属第一医院[2]Department of Spine Surgery, The First Affiliated Hospital of Dali University, Dali 671000, Yunnan, China云南省第一人民医院[3]Department of Ultrasonography, The First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan, China昆明医科大学附属第一医院[4]Department of Spine Surgery, Honghui Hospital, Xi’an Jiaotong University, No.76 Nanguo Rd., Xi’an City 710054, Shanxi, China
It is increasingly recognized that microRNAs (miRNAs) are a kind of important regulators, which are involved in the pathogenesis and development of various human diseases. However, the underlying effects and mechanism of miR-142-5p on the osteoclast differentiation of bone marrow-derived macrophages (BMMs) have not been elucidated. The aim of the present study is to explore the molecular mechanisms that regulate the osteoclastogenesis of BMMs for providing more efficient methods for treating bone-related diseases. In the present study, BMMs were isolated from rats and cultured. Moreover, receptor activators of NF-kB ligands were used to induce the osteoclast differentiation of BMMs. Furthermore, we analyzed the effects of miR-142-5p mimics/inhibitor on the osteoclastogenesis of BMMs. The results indicated that the downregulation of miR-142-5p inhibited the osteoclastogenesis of BMMs, whereas the overexpression enhanced this process. PTEN was testified to be a direct target of miR-142-5p, and its effects on the osteoclastogenesis were also described. Most importantly, treatment of LY29004 (an inhibitor of the PI3k/Akt pathway) can attenuate miR-142-5p osteoclastogenesis effects, while the inhibition effects of LY29004 on the osteoclastogenesis were abolished by knockdown of FoxO1. Taken together, our findings demonstrated that miR-142-5p promotes the osteoclastogenesis of BMMs through PI3k/Akt/FoxO1 pathway via targeting PTEN.
基金：
This work was funded by the National Natural
Science Foundation of China (Grant Nos. 81760029 and 81760030),
the Health Science and Technology Project of Yunnan province (Grant
Nos. 2016NS021, 2017NS021, 2017NS022), Ph.D. Scientific Research
Fund of the First Affiliated Hospital of Kunming Medical University
(Grant No. 2017BS030) and Scientific and Technological Innovation
Team for Spine Degenerative Disease Research of Kunming Medical
University (Grant No. CXTD201805).
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2020]版：
大类|3 区医学
小类|4 区内分泌学与代谢4 区医学：研究与实验
最新[2023]版：
大类|3 区医学
小类|3 区医学：研究与实验4 区内分泌学与代谢
JCR分区：
出版当年[2019]版：
Q3ENDOCRINOLOGY & METABOLISMQ3MEDICINE, RESEARCH & EXPERIMENTAL
最新[2023]版：
Q3ENDOCRINOLOGY & METABOLISMQ3MEDICINE, RESEARCH & EXPERIMENTAL
第一作者机构：[1]Department of Orthopaedics, The First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan, China
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
Zhenkai Lou,Zhi Peng,Bing Wang,et al.miR-142-5p promotes the osteoclast differentiation of bone marrow-derived macrophages via PTEN/PI3K/AKT/FoxO1 pathway[J].JOURNAL OF BONE AND MINERAL METABOLISM.2019,37(5):815-824.doi:10.1007/s00774-019-00997-y.
APA：
Zhenkai Lou,Zhi Peng,Bing Wang,Xingguo Li,Xing Li&Xinliang Zhang.(2019).miR-142-5p promotes the osteoclast differentiation of bone marrow-derived macrophages via PTEN/PI3K/AKT/FoxO1 pathway.JOURNAL OF BONE AND MINERAL METABOLISM,37,(5)
MLA：
Zhenkai Lou,et al."miR-142-5p promotes the osteoclast differentiation of bone marrow-derived macrophages via PTEN/PI3K/AKT/FoxO1 pathway".JOURNAL OF BONE AND MINERAL METABOLISM 37..5(2019):815-824
