机构：[1]Third Ward of Hepatobiliary and Pancreatic Surgery, the Second Affiliated Hospital of Kunming Medical University, Kunming City, Yunnan Province, China[2]Department of Hepatopancreatobiliary Surgery, The First People’s Hospital of Yunnan Province, Kunming City, Yunnan Province, China云南省第一人民医院[3]The Affiliated Hospital of Kunming University of Science and Technology, Kunming City, Yunnan Province, China云南省第一人民医院[4]Second Ward of Hepatobiliary and Pancreatic Surgery, Second Affiliated Hospital of Kunming Medical University, Kunming City, Yunnan Province, China[5]Department of Oncology, The First Affiliated Hospital of Kunming Medical University, Kunming city, Yunnan, China内科科室肿瘤内科昆明医科大学附属第一医院[6]Department of 1 Hepatobiliary and Pancreatic Surgery, the Second Affiliated Hospital of Kunming Medical University, Kunming City, Yunnan Province, China
OBJECTIVE: This study was probed to uncover the mechanism of miR-142-5p in septic liver injury. MATERIALS AND METHODS: In this study, in-vitro and in-vivo models of sepsis were used. For In-vitro sepsis model, hepatocyte cell line (L02 cells) was treated with LPS (I i popolysaccharide). Whereas for in-vivo sepsis model, cecal ligation and puncture were performed in mice. Mice were assigned into three groups: control, CLP (Cecal Ligation Puncture), CLP + miR-142-5p inhibitor group. Liver injury was assessed via H&E staining. IL-6, TNF-alpha, and IL-1 beta expressions were assayed through ELISA kits. C-caspase-9, C-caspase-3, ERK, p65, and I kappa B alpha expressions were determined via western blot and RT-qPCR. Apoptosis in LPS-induced L02 cells was detected by TUNEL staining. RESULTS: Our results show that miR-142-5p exhibited perspicuous upregulation in CLP mice tissues and LPS-induced L02 cells. On the other hand, inhibition of miR-142-5p could promote LPS-induced L02 cell activity and reduce apoptosis and inflammation. In terms of molecular mechanism, downregulation of miR-142-5p could abate sepsis-mediated acute hepatic injury by targeting SOCS1, through ERK and NF-kappa B pathway. CONCLUSIONS: Overall our results demonstrate that miR-142-5p inhibitors can mitigate septic liver injury by downregulating the inflammation and apoptosis via targeting SOCS1. Thus, miR-142-5p can serve a potential therapeutic target for sepsis mediated acute hepatic injury.
基金：
1. Yunnan Medical Leaders Foundation (No. L-2017016). 2.
Yunnan Provincial Department of Science and technology
and Kunming Medical University 2018FE001(-040).
第一作者机构：[1]Third Ward of Hepatobiliary and Pancreatic Surgery, the Second Affiliated Hospital of Kunming Medical University, Kunming City, Yunnan Province, China
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
Ning Xu,Yesheng Chen,Hong Zhu,et al.Upregulation of miR-142-5p induced by lipopolysaccharide contributes to apoptosis of hepatocytes and hepatic failure[J].EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES.2021,25(16):5293-5303.doi:10.26355/eurrev_202108_26550.
APA：
Ning Xu,Yesheng Chen,Hong Zhu,Weisi Li,Z.-W. SUN...&Yitao Bai.(2021).Upregulation of miR-142-5p induced by lipopolysaccharide contributes to apoptosis of hepatocytes and hepatic failure.EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES,25,(16)
MLA：
Ning Xu,et al."Upregulation of miR-142-5p induced by lipopolysaccharide contributes to apoptosis of hepatocytes and hepatic failure".EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES 25..16(2021):5293-5303
