Thyroid cancer is the most widespread endocrine malignant cancer and its worldwide occurrence rates are swiftly mounting more pressure in the research institutions. Even though the transience of thyroid cancer is comparatively short, its rate of reappearance is moderately elevated, which is a major cause of the incurable disease of the thyroid gland. Consequently, we used natural component which restrains the cancer cell growth by amending precise molecular mechanisms. In this current study, we assessed sesame, which is a very active substance that inhibits cell proliferation by modulation of JAK/STAT-3 signaling in thyroid cancer cell lines (FTC-133). Results showed that sesame triggers cytotoxicity, ROS production and apoptosis in FTC-133 cell lines by time-dependent approach. Furthermore, Signal Transducer and Activating Transcription (STAT-3) is a novel oncogenic transcriptional factor which normalizes apoptosis and proliferation. The reticence of STAT-3 transcription was measured as an innovative approach to hamper thyroid cancer cell growth. In this current study, we established that sesame deactivates STAT-3 translocation, thereby reducing the elevated expression of, cyclin-D1, Bcl-2 and diminished expression of Bax, caspase-9 and 3 in FTC-133 cell lines. Conclusively, sesame hampers thyroid cell expansion and initiates apoptosis by hindering STAT-3 translocation.