机构：[a]Guangdong Eye Institute, Department of Ophthalmology, Guangdong Provincial People’s Hospital, Guangdong Academy of Medical Sciences, Guangzhou, China研究所眼科广东省眼病防治研究所广东省人民医院[b]Department of Ophthalmology, General Hospital of Guangzhou Military Command of PLA, Guangzhou, China[c]Southern Medical University, Guangzhou, China[d]Department of Ophthalmology, The First Affiliated Hospital of Kunming Medical College, Kunming, China外科科室眼科昆明医科大学附属第一医院云南省第一人民医院[e]Shantou University medical college, Shantou, China[f]State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China
Age-related macular degeneration (AMD) is the most common cause of vision loss in the aged population. Aging and inflammation are thought to promote AMD pathogenesis in people with genetic predisposition. Follicular helper T (Tfh) cells play critical roles in inflammatory responses. Here, we investigated circulating Tfh cells in AMD patients. Circulating Tfh cells were defined as CXCR5(+) CD4 T cells. Data showed that patients with the wet-type AMD presented significantly higher levels of Tfh cells than non-AMD controls. Interestingly, the Tfh cells from dry and wet AMD patients also presented significantly higher ICOS and PD-1 expression, together with higher IL-17 and IL-21 expression directly ex vivo and following PMA/ionomycin stimulation. The expression of IFNg and IL-10, on the other hand, was not different between Tfh cells from AMD patients and their counterparts in non-AMD controls. Functional analysis revealed that Tfh cells from AMD patients were better at inducing the production of IgG and IgA, and this effect was in an IL-21-dependent manner. Together, we demonstrated that the circulating Tfh cell responses were dysregulated in AMD patients.
基金：
This work was supported by Grant 81870663 from the National
Natural Science Foundation of China (H.Y.), the Talent Introduction
Fund of Guangdong General hospital (Y012018145) (H.Y.), Grant
81760175 from the National Natural Science Foundation of China (L.
Y.), Grant 2016FB126 from the Applied Basic Research Foundation of
the Department of Science and Technology of Yunnan Province,
Yunnan, China (L. Y.), Grant 2014RA009 from the Department of
Science and Technology of Yunnan Province, Yunnan, China (L. Y.).
第一作者机构：[a]Guangdong Eye Institute, Department of Ophthalmology, Guangdong Provincial People’s Hospital, Guangdong Academy of Medical Sciences, Guangzhou, China[b]Department of Ophthalmology, General Hospital of Guangzhou Military Command of PLA, Guangzhou, China[c]Southern Medical University, Guangzhou, China
共同第一作者：
通讯作者：
通讯机构：[*1]Guangdong Eye Institute, Department of Ophthalmology, Guangdong Provincial People’s Hospital, Guangdong Academy of Medical Sciences, Guangzhou, China.
推荐引用方式(GB/T 7714)：
Qiaowei Wu,Baoyi Liu,Ling Yuan,et al.Dysregulations of follicular helper T cells through IL-21 pathway in age-Check forelated macular degeneration[J].MOLECULAR IMMUNOLOGY.2019,114:243-250.doi:10.1016/j.molimm.2019.07.028.
APA：
Qiaowei Wu,Baoyi Liu,Ling Yuan,Qingsheng Peng,Lin Cheng...&Honghua Yu.(2019).Dysregulations of follicular helper T cells through IL-21 pathway in age-Check forelated macular degeneration.MOLECULAR IMMUNOLOGY,114,
MLA：
Qiaowei Wu,et al."Dysregulations of follicular helper T cells through IL-21 pathway in age-Check forelated macular degeneration".MOLECULAR IMMUNOLOGY 114.(2019):243-250
