机构：[1]Department of Anesthesiology, First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan Province, China外科科室麻醉手术科（医技）昆明医科大学附属第一医院[2]Department of Anesthesiology, Kunming Angel Women’s & Children’s Hospital, Kunming, Yunnan Province, China
Background: Dexmedetomidine is widely used for perioperative and ICU patients. microRNAs (miRNAs) function as regulators of gene expression. The aim of the study was to assay expression profiling of microRNA in rat hearts following administration of dexmedetomidine. Methods: In this study 6 rats were randomly divided into two groups (n=3): dexmedetomidine group and control group. The rats of dexmedetomidine group were intraperitoneally given dexmedetomidine in a dose of 100 mu g/kg whereas the rats in control group were administered normal saline intraperitoneally. The hearts were excised 30 min after the administration of dexmedetomidine or normal saline under anesthesia. The samples were analyzed for differentially expressed microRNAs with Exiqon miRNA Array. The differentially expressed microRNAs were confirmed by using qRT-PCR. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed to find the target genes and signaling pathways of the aberrantly expressed miRNAs. Results: Six microRNAs were identified to be significantly expressed, among of which, five microRNAs (miRNA-434-3p, miRNA-3596d, miRNA-496-5p, miRNA-7a-2-3p and miRNA-702-3p) were up-regulated and 1 microRNA (miRNA-208b-3p) down-regulated compared to those of control group. The aberrantly expressed microRNAs were further validated by Quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). GO and KEGG analyses were used to identify target genes and the signaling pathways. Conclusions: The use of dexmedetomidine is associated with differentially expressed microRNAs which may be involved in cardioprotection following administration of dexmedetomidine.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China [81860050, 81460044, 81160035]; Yunnan Provincial Science and Technology Department [2017FE468 (-039)]; Health and Family Planning Commission of Yunnan Province [2018NS0126]; Program for Innovative Research Team of Kunming Medical University [CXTD201802]; Yunnan Provincial Ten Thousand-Talent Program-Famous Doctor
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2020]版：
大类|2 区医学
小类|2 区药学3 区医学：研究与实验
最新[2023]版：
大类|2 区医学
小类|1 区药学2 区医学：研究与实验
JCR分区：
出版当年[2019]版：
Q1PHARMACOLOGY & PHARMACYQ1MEDICINE, RESEARCH & EXPERIMENTAL
最新[2023]版：
Q1MEDICINE, RESEARCH & EXPERIMENTALQ1PHARMACOLOGY & PHARMACY
第一作者机构：[1]Department of Anesthesiology, First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan Province, China
共同第一作者：
通讯作者：
通讯机构：[*1]Department of Anesthesiology, First Affiliated Hospital of Kunming Medical University, #295 Xichang Road, Kunming, Yunnan Province, 650032, China.
推荐引用方式(GB/T 7714)：
Wang Lingyan,Tang Shumiao,Wang Zhuoran,et al.The administration of dexmedetomidine changes microRNA expression profiling of rat hearts[J].BIOMEDICINE & PHARMACOTHERAPY.2019,120:doi:10.1016/j.biopha.2019.109463.
APA：
Wang, Lingyan,Tang, Shumiao,Wang, Zhuoran,Chen, Hongmei,Rajcha, Shiva Sunder&Qian, Jinqiao.(2019).The administration of dexmedetomidine changes microRNA expression profiling of rat hearts.BIOMEDICINE & PHARMACOTHERAPY,120,
MLA：
Wang, Lingyan,et al."The administration of dexmedetomidine changes microRNA expression profiling of rat hearts".BIOMEDICINE & PHARMACOTHERAPY 120.(2019)
医学