Research on the changes in balance motion behavior and learning, as well as memory abilities of rats with multiple cerebral concussion-induced chronic traumatic encephalopathy and the underlying mechanism
机构:[1]College of Forensic Medicine, Kunming Medical University, Kunming, Yunnan 650500[2]Department of Medical Imaging, The First Affiliated Hospital ofKunming Medical University, Kunming, Yunnan 650032, P.R. China医技科室医学影像中心昆明医科大学附属第一医院[3]Department of Neurosurgery, The First Affiliated Hospital ofKunming Medical University, Kunming, Yunnan 650032, P.R. China外科科室神经外科神经外一科(神经外科)昆明医科大学附属第一医院
To study the effects of multiple cerebral concussion (MCC)-induced chronic traumatic encephalopathy on balance motion behavior learning and memory abilities of rats and its possible mechanism. 4MCC rat models were established by means of striking the head (4MCC group, n=15), while normal Sprague-Dawley (SD) rats were used as controls (C group, n=15). At 2 weeks after injury, balance beam (BB) test, beam walking (BW) test and Morris water maze (MWM) test were performed, respectively. The metabolites in brain tissues of rats, the number of glial fibrillary acidic protein (GFAP)-positive cells and apoptotic cells in brain slices of rats, and the expression levels of phosphorylated tau (p-tau) and A(1-40) proteins were detected. The score of rats in 4MCC group was significantly lower than that in C group (p<0.01). The escape latencies of rats in 4MCC group on the 4th-7th days during training and the time reaching the platform were significantly longer (p<0.05), but the residence time in the target quadrant was obviously shorter (p<0.01). Naphthalene acetic acid (NAA) and creatinine (Cr) values in septal coronal section in 4MCC group were significantly lower, but choline (Cho) and myo-inositol (MI) values were obviously higher (p<0.01). The number of GFAP-positive cells in the hippocampal and septal areas in 4MCC group were significantly larger (p<0.01). In the hippocampal and septal areas of 4MCC group, the number of apoptotic cells was obviously larger (p<0.01), and the expression levels of p-tau and A(1-40) proteins were significantly higher (p<0.01). Thus, MCC-induced chronic traumatic encephalopathy can increase the expressions of p-tau and A(1-40) proteins in the hippocampal and septal areas, leading to damage of hippocampal and septal neurons and increasing the number of astrocytes in the hippocampal and septal areas, ultimately damaging the balance motion behavior and learning, as well as memory abilities of rats.
基金:
National Natural Science FundNational Natural Science Foundation of China [81360467]
第一作者机构:[1]College of Forensic Medicine, Kunming Medical University, Kunming, Yunnan 650500
通讯作者:
通讯机构:[*1]College of Forensic Medicine, Kunming Medical University, 1168 West Chunrong Road, Kunming, Yunnan 650500, P.R. China
推荐引用方式(GB/T 7714):
Zhang Huan,Zhang Zhenguang,Wang Zhen,et al.Research on the changes in balance motion behavior and learning, as well as memory abilities of rats with multiple cerebral concussion-induced chronic traumatic encephalopathy and the underlying mechanism[J].EXPERIMENTAL AND THERAPEUTIC MEDICINE.2018,16(3):2295-2302.doi:10.3892/etm.2018.6474.
APA:
Zhang, Huan,Zhang, Zhenguang,Wang, Zhen,Zhen, Yongjiang,Yu, Jiangyun&Song, Hai.(2018).Research on the changes in balance motion behavior and learning, as well as memory abilities of rats with multiple cerebral concussion-induced chronic traumatic encephalopathy and the underlying mechanism.EXPERIMENTAL AND THERAPEUTIC MEDICINE,16,(3)
MLA:
Zhang, Huan,et al."Research on the changes in balance motion behavior and learning, as well as memory abilities of rats with multiple cerebral concussion-induced chronic traumatic encephalopathy and the underlying mechanism".EXPERIMENTAL AND THERAPEUTIC MEDICINE 16..3(2018):2295-2302