机构:[1]Scientific Research Experiment Center, Kunming Medical University, No. 295, Xichang Road, 65, Kunming,China[2]Department of Hepatobiliary Surgery, The First Affiliated Hospital of Kunming Medical University,Kunming, China外科科室普通外科普通外二科(肝胆外科)昆明医科大学附属第一医院[3]Kunming biological diversity regional center of instruments, Kunming Institute of Zoology, ChineseAcademy of Sciences, Kunming, China
Background: Mucin plays an essential role in the intrahepatic stone formation, but the mechanism of mucin regulation is unclear. Objective: To investigate the potential implication of miR-93 and WNT pathway in the regulation of intrahepatic bile duct mucin expression. Methods: Thirty patients with or without intrahepatic bile duct stones are involved; Reverse-transcription polymerase chain reaction was performed to evaluate the expression of MUC3, MUC4, MUC5B, MUC5AC mRNA and miR-93 levels. miR-NC or miR-93 mimics was transfected into intrahepatic biliary epithelial cells. Then mucins and Wnt pathway proteins were detected by the immunoblotting, and the target gene TCF7 were validated using the dual luciferase assay. beta-catenin, wnt4, and mucins were an immunohistochemical stain of the intrahepatic biliary epithelial tissues. Results: The expression levels of MUC3, MUC4, MUC5B, and MUC5AC in patients with intrahepatic bile duct stones are higher than control, as well as Wnt pathway proteins (especially beta-catenin and wnt4). Mucins levels increased in wnt4, wnt5a or SB216763-treated HIBECs, and reduced by miR-93 mimics transfection. miR-93 directly targeted TCF7 and repressed Wnt pathway protein expression, which reversed the upregulation of mucin levels induced by wnt4 or wnt5a, but not SB216763. Conclusion: These results suggest a new potential mechanism in intrahepatic stones, regulating by miR-93/TCF7, non-canonical Wnt pathway, and mucins. (C) 2018 Elsevier Masson SAS. All rights reserved.
基金:
Applied Basic Research Foundation of Yunnan Province, China [2015FB028]
第一作者机构:[1]Scientific Research Experiment Center, Kunming Medical University, No. 295, Xichang Road, 65, Kunming,China
通讯作者:
推荐引用方式(GB/T 7714):
Ma Hua,Li Wenyi,Bi Pinduan,et al.hsa-miR-93 regulates MUCIN family gene expression via WNT/beta-catenin pathway in intrahepatic stone disease[J].CLINICS AND RESEARCH IN HEPATOLOGY AND GASTROENTEROLOGY.2018,42(5):453-461.doi:10.1016/j.clinre.2018.03.013.
APA:
Ma, Hua,Li, Wenyi,Bi, Pinduan,Wang, Qiang,Li, Jian&Yang, Bin.(2018).hsa-miR-93 regulates MUCIN family gene expression via WNT/beta-catenin pathway in intrahepatic stone disease.CLINICS AND RESEARCH IN HEPATOLOGY AND GASTROENTEROLOGY,42,(5)
MLA:
Ma, Hua,et al."hsa-miR-93 regulates MUCIN family gene expression via WNT/beta-catenin pathway in intrahepatic stone disease".CLINICS AND RESEARCH IN HEPATOLOGY AND GASTROENTEROLOGY 42..5(2018):453-461