Background/Aims: Circulating miRNAs could serve as biomarkers for diagnosis or prognosis of heart diseases and cerebrovascular diseases. Dexmedetomidine has protective effects in various organs. The effects of dexmedetomidine on circulating miRNAs remain unknown. Here, we investigated differentially expressed miRNA and to predict the target genes of the miRNA in patients receiving dexmedetomidine. Methods: The expression levels of circulating miRNAs of 3 patients were determined through high through-put miRNA sequencing technology. Target genes of the identified differentially expressed miRNAs were predicted using TargetScan 7.1 and miRDB v.5. Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) were used to conduct functional annotation and pathway enrichment analysis of target genes respectively. Results: Twelve differentially expressed miRNAs were identified. Five miRNAs were upregulated (hsa-miR-4508, hsa-miR-novel-chr8_87373, hsa-miR-30a-3p, hsa-miR-novel-chr16_26099, hsa-miR-4306) and seven miRNAs (hsa-miR-744-5p, hsa-miR-320a, hsa-miR-novel-chr9_90035, hsa-miR-101-3p, hsa-miR-150-5p, hsa-miR-342-3p, and hsa-miR-140-3p) were downregulated after administration of dexmedetomidine in the subjects. The target genes and pathways related to the differentially expressed miRNAs were predicted and analyzed. Conclusion: The differentially expressed miRNAs may be involved in the mechanisms of action of dexmedetomidine. Specific miRNAs, such as hsa-miR-101-3p, hsa-miR-150-5p and hsa-miR-140-3p, are new potential targets for further functional studies of dexmedetomidine. (c) 2018 The Author(s) Published by S. Karger AG, Basel
基金:
National Natural Science Foundation of ChinaNational Natural Science Foundation of China [81460044, 81860050, 81160035]; Yunnan Provincial Science and Technology Department [2014HB031, 2017FE468 (-039)]; Health and Family Planning Commission of Yunnan Province [2018NS0126]; Program for Innovative Research Team of Kunming Medical University [CXTD201802]; Yunnan health training project of high level talents
第一作者机构:[1]Kunming Med Univ, Affiliated Hosp 1, Dept Anesthesiol, 295 Xichang Rd, Kunming 650032, Yunnan, Peoples R China;
共同第一作者:
通讯作者:
通讯机构:[1]Kunming Med Univ, Affiliated Hosp 1, Dept Anesthesiol, 295 Xichang Rd, Kunming 650032, Yunnan, Peoples R China;
推荐引用方式(GB/T 7714):
Yang Xuehui,Chen Hongmei,Chen Yan,et al.Circulating miRNA Expression Profiling and Target Prediction in Patients Receiving Dexmedetomidine[J].CELLULAR PHYSIOLOGY AND BIOCHEMISTRY.2018,50(2):552-568.doi:10.1159/000494168.
APA:
Yang, Xuehui,Chen, Hongmei,Chen, Yan,Birnbaum, Yochai,Liang, Rongbi...&Qian, Jinqiao.(2018).Circulating miRNA Expression Profiling and Target Prediction in Patients Receiving Dexmedetomidine.CELLULAR PHYSIOLOGY AND BIOCHEMISTRY,50,(2)
MLA:
Yang, Xuehui,et al."Circulating miRNA Expression Profiling and Target Prediction in Patients Receiving Dexmedetomidine".CELLULAR PHYSIOLOGY AND BIOCHEMISTRY 50..2(2018):552-568