Role of Wnt/beta-catenin signaling pathway in the repair of intestinal mucosa associated with crypt stem cell in a rat model of abdominal compartment syndrome
The Wnt/beta-catenin signaling plays a vital role in the maintenance of intestinal crypt epithelial cell regeneration, but its role in intestinal epithelial cell after injury is poorly understood. This study investigated the role of Wnt/beta-catenin signaling on the regeneration of intestinal crypt stem cells in Abdominal Compartment Syndrome (ACS) rat model. Arterial blood was collected for biochemical analysis and then microscopic observation showed extensive ileal villus damage after prolonged exposure to Intra-abdominal pressure. Moreover, ACS injury increased the mRNA and protein expressions for Wnt-1, beta-catenin, transcription factor 4 (TCF4), and c-Myc. Immunofluorescence staining confirmed the increased proteins of beta-catenin and c-Myc in the intestinal crypt stem cells according to morphology. Liver, kidney, heart and other organs were injured after ACS indicated by the blood biochemical analysis, and ACS causes the intestinal mucosal injury, which aggravates with time, and the Wnt/beta-catenin signaling pathway is involved in intestinal epithelium regeneration, which is associated with crypt stem cells in early stage after ACS injury.
基金：
Foundation of Medical Leading Talent of Yunnan Province [L-201205]; Foundation of Yunnan Institute of Digestive Disease [2014NS122, 2014NS124]; Yunnan Provincial Science and Technology Department and Kunming medical University [2015FB024]; Kunming Medical University Postgraduate Innovation Fund [2016S12]
第一作者机构：[1]Kunming Med Univ, First Affiliated Hosp, Dept Gastrointestinal Surg, Kunming, Peoples R China;
通讯作者：
通讯机构：[1]Kunming Med Univ, First Affiliated Hosp, Dept Gastrointestinal Surg, Kunming, Peoples R China;[3]Inst Neurosci Kunming Med Univ, Anim Ctr, Kunming, Peoples R China
推荐引用方式(GB/T 7714)：
Lu Tong,Luo Huayou,Liu Jia,et al.Role of Wnt/beta-catenin signaling pathway in the repair of intestinal mucosa associated with crypt stem cell in a rat model of abdominal compartment syndrome[J].INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY.2017,10(2):2351-2362.
APA：
Lu, Tong,Luo, Huayou,Liu, Jia,Chen, Cheng,Wang, Feng...&Wang, Kun-Hua.(2017).Role of Wnt/beta-catenin signaling pathway in the repair of intestinal mucosa associated with crypt stem cell in a rat model of abdominal compartment syndrome.INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY,10,(2)
MLA：
Lu, Tong,et al."Role of Wnt/beta-catenin signaling pathway in the repair of intestinal mucosa associated with crypt stem cell in a rat model of abdominal compartment syndrome".INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY 10..2(2017):2351-2362
