机构：[1]Department of Hematology, The Second Affiliated Hospital of Kunming Medical University, Kunming 650101, China[2]Department of Hematology, The First Affiliated Hospital of Kunming Medical University, Kunming 650041, China内科科室血液内科昆明医科大学附属第一医院
Objective(s): The aim of this study was to investigate the methylation status and mRNA expression levels of P-15, death-associated protein kinase (DAPK), and suppressor of cytokine signaling-1 (SOCS1) genes in multiple myeloma (MM). Materials and Methods: The bone marrow samples of 54 MM patients were collected and the methylation status of the P-15, DAPK, and SOCS1 gene promoter regions was determined by methylation-specific polymerase chain reaction. Automated sequencing technology was used to sequence the amplified products in order to analyze the base methylation sites. mRNA expression levels were determined using real-time fluorescent quantitative polymerase chain reaction. Results: Among the 54 MM patients, the positive methylation rates of the P-15, DAPK, and SOCS1 genes were 27.78%, 18.52%, and 16.67%, respectively. The methylation results were confirmed by sequencing. The positive methylation rates of the P-15, DAPK, and SOCS1 genes showed no correlation with patient gender, age, typing, staging, and grouping (P>0.05). There was no significant difference in the mRNA expression levels of the P-15, DAPK, and SOCS1 genes between the MM patient group and the control group (P>0.05). Conclusions: Aberrant methylation of the P-15, DAPK, and SOCS1 genes exists in MM, and these genes may play certain roles in pathogenesis of MM. There was no significant difference in mRNA expression levels between the methylated group and the non-methylated group, suggesting that these genes are regulated by other mechanisms during their transcription.
基金：
Projects of Yunnan basic application researches [2009CD172]
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2017]版：
大类|4 区医学
小类|4 区医学：研究与实验4 区药学
最新[2023]版：
大类|4 区医学
小类|4 区医学：研究与实验4 区药学
JCR分区：
出版当年[2016]版：
Q3MEDICINE, RESEARCH & EXPERIMENTALQ4PHARMACOLOGY & PHARMACY
最新[2023]版：
Q3MEDICINE, RESEARCH & EXPERIMENTALQ3PHARMACOLOGY & PHARMACY
第一作者机构：[1]Department of Hematology, The Second Affiliated Hospital of Kunming Medical University, Kunming 650101, China[*1]Department of Hematolo gy, The Second Affiliated Hospital of Kunming Medical University, Kunming 650101, China
通讯作者：
通讯机构：[*1]Department of Hematolo gy, The Second Affiliated Hospital of Kunming Medical University, Kunming 650101, China
推荐引用方式(GB/T 7714)：
Liu Lin,Tan Lin,He Zhenxin.Methylation and mRNA expression levels of P-15, death-associated protein kinase, and suppressor of cytokine signaling-1 genes in multiple myeloma[J].IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES.2016,19(7):755-762.
APA：
Liu, Lin,Tan, Lin&He, Zhenxin.(2016).Methylation and mRNA expression levels of P-15, death-associated protein kinase, and suppressor of cytokine signaling-1 genes in multiple myeloma.IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES,19,(7)
MLA：
Liu, Lin,et al."Methylation and mRNA expression levels of P-15, death-associated protein kinase, and suppressor of cytokine signaling-1 genes in multiple myeloma".IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES 19..7(2016):755-762
