机构：[1]Department of Biomedical Engineering,College of Engineering and Applied Sciences,Nanjing University,Nanjing,Jiangsu 210093,China[2]Department of Nanomedicine,Houston Methodist Research Institute,6670 BertnerAve,Houston,TX77030,USA[3]Department of Celland DevelopmentalBiology,WeillMedical Collegeof Cornell University,NewYork,NY10065,USA[4]Department of Obstetrics & Gynecology,The First Affiliated Hospital of Kunming Medical University,Kunming,Yunnan660032,China外科科室妇产科产科妇科昆明医科大学附属第一医院
Platinum nanoparticles incorporated volumetric bar-chart chip (PtNPs-V-Chip) is able to be used for point-of-care tests by providing quantitative and visualized readout without any assistance from instruments, data processing, or graphic plotting. To improve the sensitivity of PtNPs-V-Chip, hybridization chain reaction was employed in this quantitation platform for highly sensitive assays that can detect as low as 16 pM Ebola Virus DNA, 0.01 ng/mL carcinoembryonic antigen (CEA), and the 10 HER2-expressing cancer cells. Based on this amplified strategy, a 100-fold decrease of detection limit was achieved for DNA by improving the number of platinum nanoparticle catalyst for the captured analyte. This quantitation platform can also distinguish single base mismatch of DNA hybridization and observe the concentration threshold of CEA. The new strategy lays the foundation for this quantitation platform to be applied in forensic analysis, biothreat detection, clinical diagnostics and drug screening. (C) 2016 Elsevier B.V. All rights reserved.
基金：
Thousand Talents Program for Young Researchers; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [81160076]; Fostering Talents Project for Young Academic and Technology leaders in Yunnan Province [2012HB029]
第一作者机构：[1]Department of Biomedical Engineering,College of Engineering and Applied Sciences,Nanjing University,Nanjing,Jiangsu 210093,China[2]Department of Nanomedicine,Houston Methodist Research Institute,6670 BertnerAve,Houston,TX77030,USA[3]Department of Celland DevelopmentalBiology,WeillMedical Collegeof Cornell University,NewYork,NY10065,USA
通讯作者：
推荐引用方式(GB/T 7714)：
Wang Yuzhen,Zhu Guixian,Qi Wenjin,et al.A versatile quantitation platform based on platinum nanoparticles incorporated volumetric bar-chart chip for highly sensitive assays[J].BIOSENSORS & BIOELECTRONICS.2016,85:777-784.doi:10.1016/j.bios.2016.05.090.
APA：
Wang, Yuzhen,Zhu, Guixian,Qi, Wenjin,Li, Ying&Song, Yujun.(2016).A versatile quantitation platform based on platinum nanoparticles incorporated volumetric bar-chart chip for highly sensitive assays.BIOSENSORS & BIOELECTRONICS,85,
MLA：
Wang, Yuzhen,et al."A versatile quantitation platform based on platinum nanoparticles incorporated volumetric bar-chart chip for highly sensitive assays".BIOSENSORS & BIOELECTRONICS 85.(2016):777-784
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