机构：[1]Department of Oncology, The First Affiliated Hospital of Kunming Medical University, Kunming 650032, People’s Republic of China内科科室肿瘤内科昆明医科大学附属第一医院[2]Department of Pathology, The First People’s Hospital of Yunnan Province, Kunming 650032, People’s Republic of China云南省第一人民医院[3]Institute of Basic Medicine of the First People’s Hospital of Yunnan Province, Center of Clinical Molecular Biology of Yunnan Province, Kunming 650032, People’s Republic of China云南省第一人民医院
As activin/nodal signaling plays a key role in definitive endoderm (DE) differentiation, we have explored activin A-induced differentiation of DE from human parthenogenetic embryonic stem cells (hPESCs). Administration of 5 ng activin A/ml had no effect on the expression of markers of DE differentiation. However, higher concentrations of activin A (50 and 100 ng/ml) upregulated Sox17 and Cxcr4, as well upregulating the mesendodermal precursor marker, Brachyury. These findings demonstrate that low dose activin A can maintain the undifferentiated potency of hPESCs, whereas higher doses induce DE differentiation; 50 ng/ml is the optimal concentration for inducing DE from hPESCs.
基金：
This study was supported by Grants from
the Applied Basic Research Project of Yunnan Province
(2013FZ179, 2014FB040).
第一作者机构：[1]Department of Oncology, The First Affiliated Hospital of Kunming Medical University, Kunming 650032, People’s Republic of China
通讯作者：
推荐引用方式(GB/T 7714)：
Zhiqiang Wang,Wenliang Li,Tianxing Chen,et al.Activin A can induce definitive endoderm differentiation from human parthenogenetic embryonic stem cells[J].BIOTECHNOLOGY LETTERS.2015,37(8):1711-1717.doi:10.1007/s10529-015-1829-x.
APA：
Zhiqiang Wang,Wenliang Li,Tianxing Chen,Jun Yang,Zhengqi Wen...&Rui Liang.(2015).Activin A can induce definitive endoderm differentiation from human parthenogenetic embryonic stem cells.BIOTECHNOLOGY LETTERS,37,(8)
MLA：
Zhiqiang Wang,et al."Activin A can induce definitive endoderm differentiation from human parthenogenetic embryonic stem cells".BIOTECHNOLOGY LETTERS 37..8(2015):1711-1717
