机构：[1]Department of Histology and Neurobiology,West China School of Preclinical and Forensic Medicine, Sichuan, Peoples R China[2]Department of SanitaryTechnology,West China School of Public Health, Sichuan University, Sichuan昆明医科大学附属第一医院[3]Institute of Research on Neuroscience,Kunming, P.R.China[4]The First A⁄liated Hospital of Kunming Medical College,Kunming, P.R.China
This investigation aimed to isolate neural stem cells from neonatal hippocampus and induce them to differentiate into cholinergic neurons. The isolated neural stem cells were incubated in serum-free Dulbecco's modified Eagle medium/F12 medium added with 20 ng/ml basic fibroblast growth factor and B27 The cell line isolated from the hippocampal formation of neonatal rats expressed nestin and had the potency to form clones and differentiate into neurons, astrocytes and oligodendrocytes. Embryonic chick skeletal muscle extract was used to induce the differentiation of the neural stem cells into cholinergic neurons. Immunocytochemistry was used to detect the choline acetyltransferase antigen of cholinergic neurons for confirmation. The results showed that embryonic chick skeletal muscle extract could induce isolated neural stem cell to differentiate into a significantly larger number of cholinergic neurons than controls.
基金：
This work was supported by a grant from the National Science Foundation of China (Grant No. 30260112, 30260125), the Natural Science Fund
of Yunnan Province (Grant No. 2003C53M, 2000C11R), the Development Grant of the Kunming Medical College (No. 2005DG02) and the Chinese Medical
Board of NewYork (CMB- 00 -72).
第一作者机构：[1]Department of Histology and Neurobiology,West China School of Preclinical and Forensic Medicine, Sichuan, Peoples R China[3]Institute of Research on Neuroscience,Kunming, P.R.China[*1]Institute of Research on Neuroscience,191West of Renmin Road, Kunming,Yunnan Province 650031, China
通讯作者：
通讯机构：[*1]Institute of Research on Neuroscience,191West of Renmin Road, Kunming,Yunnan Province 650031, China
推荐引用方式(GB/T 7714)：
Tim Ting Hua Wang,Ai-Hong Jing,Xiang-Ying Luo,et al.Neural stem cells: isolation and differentiation into cholinergic neurons[J].NEUROREPORT.2006,17(13):1433-1436.doi:10.1097/01.wnr.0000227980.06013.31.
APA：
Tim Ting Hua Wang,Ai-Hong Jing,Xiang-Ying Luo,Ming Li,Yan Kang...&Su Liu.(2006).Neural stem cells: isolation and differentiation into cholinergic neurons.NEUROREPORT,17,(13)
MLA：
Tim Ting Hua Wang,et al."Neural stem cells: isolation and differentiation into cholinergic neurons".NEUROREPORT 17..13(2006):1433-1436
