机构:[1]Department of Gastroenterology, The First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunan Province, China内科科室外科科室消化内科普通外科普通外一科(胃肠与疝外科)昆明医科大学附属第一医院[2]Department of Gastroenterology, The Second People’s Hospital of Yunnan Province, Kunming 650021, Yunan Province, China[3]Department of Clinical Laboratory, The First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunan Province, China昆明医科大学附属第一医院
AIM: To identify peripheral blood mononuclear cell (PBMC) gene expression profiles of ulcerative colitis (UC) patients, using oligonucleotide microarrays, to gain insights into UC molecular mechanisms. METHODS: The Human OneArray microarrays were used for a complete genome-wide transcript profiling of PBMCs from 12 UC patients and 6 controls. Differential analysis per gene was performed with a random variance model; t test and P values were adjusted to control the false discovery rate (5%). Gene ontology (GO) was deployed to analyze differentially expressed genes at significant levels between patients and controls to identify the biological processes involved in UC. RESULTS: Comparative analysis revealed that 4438 probes (4188 genes) were differentially expressed between the two groups, of which 3689 probes (3590 genes) were down-regulated whereas 749 probes (598 genes) were up-regulated. Many disregulated genes in our data have been reported by previous microarray studies carried out on intestinal mucosa samples, such as S100A8, CEACAM1 and S100A9. GO enrichment analysis revealed 67 high enrichment up-regulated categories and one significant down-regulated category. The up-regulated genes were mainly involved in immune and inflammatory response, cell cycle and proliferation, DNA metabolism and repair. CONCLUSION: Gene expression profiling of PBMCs from patients with UC has highlighted several novel gene categories that could contribute to the pathogenesis of UC. (C) 2013 Baishideng. All rights reserved.
基金:
National Natural Science Foundation of ChinaNational Natural Science Foundation of China [81260074/H0310, 81160055/H0310]; Confederative Special Foundation of Science and Technology, Department of Yunnan Province; Kunming Medical College [2011FB183, 2007C0010R]; Yunnan Provincial Bureau of Health [D-201215]
第一作者机构:[1]Department of Gastroenterology, The First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunan Province, China[*1]Department of Gastroenterology, The First Affiliated Hospital of Kunming Medical University, 295 Xichang road, Kunming 650032, Yunan Province, China
通讯作者:
通讯机构:[1]Department of Gastroenterology, The First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunan Province, China[3]Department of Clinical Laboratory, The First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunan Province, China[*1]Department of Gastroenterology, The First Affiliated Hospital of Kunming Medical University, 295 Xichang road, Kunming 650032, Yunan Province, China
推荐引用方式(GB/T 7714):
Miao Ying-Lei,Xiao Yu-Liang,Du Yan,et al.Gene expression profiles in peripheral blood mononuclear cells of ulcerative colitis patients[J].WORLD JOURNAL OF GASTROENTEROLOGY.2013,19(21):3339-3346.doi:10.3748/wjg.v19.i21.3339.
APA:
Miao, Ying-Lei,Xiao, Yu-Liang,Du, Yan&Duan, Li-Ping.(2013).Gene expression profiles in peripheral blood mononuclear cells of ulcerative colitis patients.WORLD JOURNAL OF GASTROENTEROLOGY,19,(21)
MLA:
Miao, Ying-Lei,et al."Gene expression profiles in peripheral blood mononuclear cells of ulcerative colitis patients".WORLD JOURNAL OF GASTROENTEROLOGY 19..21(2013):3339-3346