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LncRNA FLVCR-AS1 promotes osteosarcoma growth by targeting mir381-3p/CCND1(Open Access)

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机构： [a]Department of Orthopedics, The Second Affiliated Hospital of Soochow University, Suzhou, 215004, China [b]Department of Orthopedics, First Affiliated Hospital of Kunming Medical University, Kunming, 650200, China [c]Department of Orthopedics, Affiliated Hospital of Putian University, Putian, 351100, China

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关键词： Apoptosis CCND1 FLVCR-AS1 MiR381-3p OS Proliferation

摘要：

Purpose: This article reports on FLVCR-AS1 effects on osteosarcoma (OS) growth. Methods: Tumor tissue and adjacent normal tissue of 48 OS patients were collected. HOS and 143B cells were transfected. Gene expression was examined with qRT-PCR and Western blot. CCK8 assays and cell cloning was performed to measure cell proliferation. Cell cycle and apoptosis were assessed. Luciferase-reporter gene assays and RNA pull-down tests were used to detect targeting relationships between genes. Results: Prominently higher FLVCR-AS1 expression was found in OS tissue and cells, and was associated with poor prognosis (P<0.05, P<0.01, or P<0.001). Compared with the siCtrl group, 143B and HOS cells of the siFLVCR-AS1 group had significantly lower OD450 values and clone numbers and obviously higher percentages of cells in the G1 phase and apoptosis (P<0.01 or P<0.001). miR381-3p expression was directly inhibited by FLVCR-AS1, and CCND1 expression was directly suppressed by miR381-3p. Compared with the FLVCR-AS1 group, 143B cells of the FLVCR-AS1+ miR381-3p mimic group and FLVCR-AS1+ siCCND1 group showed remarkably lower OD450 values and clone numbers obviously higher apoptosis and percentage of cells in the G1 phase (P<0.05, P<0.01, or P<0.001). Conclusion: FLVCR-AS1 promoted OS growth by upregulating CCND1 expression via downregulation of miR381-3p. © 2020 Yang et al.

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出版当年[2021]版：

大类 | 3 区医学

小类 | 3 区生物工程与应用微生物 3 区肿瘤学

最新[2023]版：

大类 | 4 区医学

小类 | 3 区生物工程与应用微生物 4 区肿瘤学

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出版当年[2020]版：

Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Q3 ONCOLOGY

最新[2023]版：

Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Q3 ONCOLOGY

影响因子： 2.7 最新[2023版] 3.1 最新五年平均 4.147 出版当年[2020版] 3.763 出版当年五年平均 3.337 出版前一年[2019版] 4.345 出版后一年[2021版]

第一作者：

第一作者机构： [a]Department of Orthopedics, The Second Affiliated Hospital of Soochow University, Suzhou, 215004, China [b]Department of Orthopedics, First Affiliated Hospital of Kunming Medical University, Kunming, 650200, China

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