机构：[1]Department of Dermatology, the First Affiliated Hospital of Kunming Medical University, Kunming, China内科科室皮肤科昆明医科大学附属第一医院[2]Department of Physiology, Kunming Medical University, Kunming, China[3]Department of Plastic Surgery, Second Affiliated Hospital of Kunming Medical University, Kunming, China[4]Department of Orthopedics, the First People’s Hospital of Kunming, Kunming, China
microRNAs (miRNAs) play a pivotal role in the regulation of cell proliferation and apoptosis in keloid scarring. Integrative analysis of the previous miRNA microarray revealed miRNA-31 was among the most frequently altered miRNAs in keloid and hypertrophic scar. Using qRT-PCR, we further validated miRNA-31 was increased in keloid tissues and keloid-derived fibroblasts. Moreover, downregulation of miRNA-31 inhibited the cell proliferation, induced the cell apoptosis and disturbed the cell cycle progression by targeting HIF1AN, a negative modulator of hypoxia inducible factor 1. Through the luciferase reporter assay, HIF1AN was confirmed to be a target of miRNA-31. Further studies demonstrated that miRNA-31 regulated proliferation, apoptosis and cell cycle of keloid-derived fibroblasts by mediating HIF1AN/VEGF signaling pathway. Overall, our findings shed new light on miRNA-31 as a promising therapeutic target in keloid scarring.
基金：
National Natural Science Foundation of China (NSFC)National Natural Science Foundation of China [81472540, 81560502, 81660518]; Science and Technology Leading Talent Project of Yunnan province [2017HA010]; National Natural Science Foundation of Yunnan Province [2017FB116, 2014FB008]; Education Department Fund of Yunnan Province [2014Y165, 2015Z082]; 100 Talents Program of Kunming Medical University; Graduate Innovation Fund [2017B010, 2017S014]
第一作者机构：[1]Department of Dermatology, the First Affiliated Hospital of Kunming Medical University, Kunming, China[2]Department of Physiology, Kunming Medical University, Kunming, China
共同第一作者：
通讯作者：
通讯机构：[1]Department of Dermatology, the First Affiliated Hospital of Kunming Medical University, Kunming, China[2]Department of Physiology, Kunming Medical University, Kunming, China
推荐引用方式(GB/T 7714)：
Zhang Juan,Xu Dan,Li Na,et al.Downregulation of microRNA-31 inhibits proliferation and induces apoptosis by targeting HIF1AN in human keloid[J].ONCOTARGET.2017,8(43):74623-74634.doi:10.18632/oncotarget.20284.
APA：
Zhang, Juan,Xu, Dan,Li, Na,Li, Yan,He, Yongjing...&He, Li.(2017).Downregulation of microRNA-31 inhibits proliferation and induces apoptosis by targeting HIF1AN in human keloid.ONCOTARGET,8,(43)
MLA：
Zhang, Juan,et al."Downregulation of microRNA-31 inhibits proliferation and induces apoptosis by targeting HIF1AN in human keloid".ONCOTARGET 8..43(2017):74623-74634
