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MiR-519d inhibits prostate cancer cell proliferation, cycle and invasion via targeting NRBP1

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机构： [a]Department of Urology, Tangshan Gongren Hospital, Tangshan, China [b]Department of Pathology, Hospital of Heilongjiang Province, Harbin, China [c]Department of Internal Medicine, Tumor Hospital of Qingdao City, Qingdao, China [d]Department of Nephrology, First Affiliated Hospital, Kunming Medical University, Kunming, China

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关键词： Cell invasion Cell proliferation Micrornas Prostate cancer

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OBJECTIVE: To investigate the effects of miR-519d on the proliferation, cycle and invasion of human prostate cancer PC3 cells and its possible molecular mechanism. MATERIALS AND METHODS: The proliferation, cycle, and invasion of human prostate cancer PC3 cells were detected via cell counting kit-8 (CCK-8) and transwell assay. The expression of NRBP1 mRNA was detected via reverse transcription-polymerase chain reaction (RT-PCR). Western blotting was used to detect the expression of NRBP1, cyclin D1, and epithelial-mesenchymal transition (EMT) markers. RES ULTS: The expression of miR-519d in prostate cancer was decreased, which was correlated with tumor size, metastasis, and staging. Proliferation, cycle, and invasion of PC3 cells were significantly decreased after overexpression of miR-519d. Bioinformatics analysis and Western blotting showed that there was a potential miR-519d binding site in NRBP1 3'-UTR, and overexpression of miR-519d significantly inhibited the expression of NRBP1. The expression of E-cadherin in PC3 cells overexpressing miR-519d was up-regulated, and the expressions of N-cadherin, cyclin D1, vimentin, fibronectin, and Snail were down-regulated. CONCLUSIONS: MiR-519d can repress the proliferation, cycle, and invasion of prostate cancer PC3 cells by inhibiting NRBP1. © 2018 Verduci Editore s.r.l. All rights reserved.

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出版当年[2019]版：

大类 | 4 区医学

小类 | 4 区药学

最新[2023]版：

大类 | 4 区医学

小类 | 4 区药学

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第一作者机构： [a]Department of Urology, Tangshan Gongren Hospital, Tangshan, China

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