个人科研网厅 | | 帮助
高级检索
当前位置： 首页 > 详情页

Transgene expression and differentiation of baculovirus-transduced adipose-derived stem cells from dystrophin-utrophin double knock-out mouse

| 导出 | |

文献详情

资源类型：
WOS体系：
Pubmed体系：

收录情况： ◇ SCIE ◇ CSCD-C

作者：
Qiuling Li[1,2] ; Qiongxiang Zhai[2] ; Jia Geng[3] ; Hui Zheng[4] ; Fei Chen[5] ; Jie Kong[1] ; Cheng Zhang[1,*1] ;
机构： [1]Department of Neurology, First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong Province [2]Department of Pediatrics, Guangdong General Hospital, Guangdong Neuroscience Institute, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong Province [3]Department of Neurology, First Affiliated Hospital, Kunming Medical College, Kunming, Yunnan Province [4]Department of Neurology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong Province [5]Center for Stem Cell Biology and Tissue Engineering, Sun Yat-sen University, Guangzhou, Guangdong Province
出处：
DOI： 10.3969/j.issn.1673-5374
ISSN：

关键词： Adipose-derived stem cells Baculovirus Duchenne muscular dystrophy Gene therapy Microdystrophin Myogenesis Neural regeneration β-catenin

摘要：
In this study, recombinant baculovirus carrying the microdystrophin and β-catenin genes was used to infect adipose-derived stem cells from a dystrophin-utrophin double knock-out mouse. Results showed that, after baculovirus transgene infection, microdystrophin and β-catenin genes were effectively expressed in adipose-derived stem cells from the dystrophin-utrophin double knock-out mouse. Furthermore, this transgenic expression promoted adipose-derived stem cell differentiation into muscle cells, but inhibited adipogenic differentiation. In addition, protein expression related to the microdystrophin and Wnt/β-catenin signaling pathway was upregulated. Our experimental findings indicate that baculovirus can successfully deliver the microdystrophin and β-catenin genes into adipose-derived stem cells, and the microdystrophin and Wnt/β-catenin signaling pathway plays an important role in myogenesis of adipose-derived stem cells in the dystrophin-utrophin double knock-out mouse. © 2012, Editorial Board of Neural Regeneration Research. All rights reserved.

基金：
This work was supported by the National Natural Science Foundation of China, No. 30370510, 30170337, 30400322, 30870851; CMB Fund, No. 4209347; Key Project of the State Ministry of Public Health, No. 2001321; Fok Ying Tung Education Foundation, No. 91029; and Key Projects in the National Science and Technology Pillar Program During the Eleventh Five-Year Plan Period, No. 2006BAI05A07.
语种：
外文
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2013]版：
大类 | 4 区 医学
小类 | 4 区 细胞生物学 4 区 神经科学
最新[2023]版：
大类 | 2 区 医学
小类 | 3 区 细胞生物学 3 区 神经科学
JCR分区：
出版当年[2012]版：
Q4 NEUROSCIENCES Q4 CELL BIOLOGY
最新[2023]版：
Q1 NEUROSCIENCES Q2 CELL BIOLOGY

影响因子： 最新[2023版] 最新五年平均 出版当年[2012版] 出版当年五年平均 出版前一年[2011版] 出版后一年[2013版]

第一作者：
第一作者机构： [1]Department of Neurology, First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong Province [2]Department of Pediatrics, Guangdong General Hospital, Guangdong Neuroscience Institute, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong Province
通讯作者：
通讯机构： [1]Department of Neurology, First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong Province [*1]Department of Neurology, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China
推荐引用方式(GB/T 7714)：
Qiuling Li,Qiongxiang Zhai,Jia Geng,et al.Transgene expression and differentiation of baculovirus-transduced adipose-derived stem cells from dystrophin-utrophin double knock-out mouse[J].Neural Regeneration Research.2012,7(22):1695-1702.doi:10.3969/j.issn.1673-5374.
APA：
Qiuling Li,Qiongxiang Zhai,Jia Geng,Hui Zheng,Fei Chen...&Cheng Zhang.(2012).Transgene expression and differentiation of baculovirus-transduced adipose-derived stem cells from dystrophin-utrophin double knock-out mouse.Neural Regeneration Research,7,(22)
MLA：
Qiuling Li,et al."Transgene expression and differentiation of baculovirus-transduced adipose-derived stem cells from dystrophin-utrophin double knock-out mouse".Neural Regeneration Research 7..22(2012):1695-1702

相关文献

[1]Transgene expression and differentiation of baculovirus-transduced adipose-derived stem cells from dystrophin-utrophin double knock-out mouse [2]Adipose-derived stem cells enhance myogenic differentiation in the mdx mouse model of muscular dystrophy via paracrine signaling [3]Decorin promotes myogenic differentiation and mdx mice therapeutic effects after transplantation of rat adipose-derived stem cells [4]Coculture of Vascular Endothelial Cells and Adipose-Derived Stem Cells as a Source for Bone Engineering [5]Directed differentiation of motor neuron cell-like cells from human adipose-derived stem cells in vitro [6]Effects of Tissue-engineered Bone by Coculture of Adipose-derived Stem Cells and Vascular Endothelial Cells on Host Immune Status. [7]Knockdown of MiR-140-5 promotes osteogenesis of adipose-derived mesenchymal stem cells by targeting TLR4 and BMP2 and promoting fracture healing in the atrophic nonunion rat model [8]Improvement of the survival of human autologous fat transplantation by adipose-derived stem-cells-assisted lipotransfer combined with bfgf(Open Access) [9]Exosomes from miR-29a-modified adipose-derived mesenchymal stem cells reduce excessive scar formation by inhibiting TGF-beta 2/Smad3 signaling [10]血管内皮细胞和脂肪干细胞体外联合培养细胞的增殖

资源点击量：52537 今日访问量：0 总访问量：1562 更新日期：2024-09-01 建议使用谷歌、火狐浏览器 常见问题

版权所有©2020 昆明医科大学第一附属医院 技术支持：重庆聚合科技有限公司 地址：云南省昆明市西昌路295号(650032)