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MiR145 regulates the proliferation and apoptosis of rat vascular endothelial cells under hyperglycemia by targeting the ANGPT2 gene and involving the NFκB signaling pathway(Open Access)

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WOS体系：
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收录情况： ◇ SCIE

作者：
Zhang, W[a] ; Tang, X.-H[b] ; Zhang, J.-J[c] ; He, Q[d] ;
机构： [a]Clinical Medical Research Center and Yunnan Provincial Key Laboratory of Clinical Virology (2018DG010), First People’s Hospital of Yunnan Province, Affiliated Hospital of Kunming University of Science and Technology, Kunming, Yunnan 650032, China [b]Center of Genetic Diagnosis, First People’s Hospital of Yunnan Province, Affiliated Hospital of Kunming University of Science and Technology, Kunming, Yunnan 650032, China [c]Kunming Institute of Zoology, Chinese Academy of Science (CAS), Kunming, Yunnan 650223, China [d]Emergency Department, First People’s Hospital of Yunnan Province, Affiliated Hospital of Kunming University of Science and Technology, Kunming, Yunnan 650032, China
出处：
DOI： 10.2147/DMSO.S273451
ISSN：

关键词： Angiopoietin 2 ANGPT2 Diabetic vasculopathy Hyperglycemia MiR145 RAECs Rat aortic endothelial cells

摘要：
Purpose: A majority of diabetes mellitus patients with disturbances of glucose metabolism present with vascular complications. This study aimed to explore regulatory mechanisms of miR145 and its potential target gene ANGPT2 on diabetic vasculopathy under hyperglycemia. Methods: Based on the fact that miR145 is detected in rat aortic endothelial cells (RAECs) under hyperglycemia, RAECs were transfected with miR145 mimics/inhibitor for further confirmation. RAEC proliferation was detected with CCK8 assays, and cell apoptosis and CD34+-cell population with annexinV–PI staining and anti-CD34FITC on flow cytometry, respectively. Then, qPCR and Western blot were applied to detect mRNA and protein expression of ANGPT2 and involved pathway factor NFκB p65. Subsequently, dual lucifer-ase–reporter gene analysis was utilized to verify whether miR145 acted directly upon the 3ʹUTR of ANGPT2 mRNA. Results: The ANGPT2 gene was confirmed to be a direct target of miR145. miR145 mimics markedly downregulated the expression of ANGPT2 and NFκB p65, boosted the percentage of the CD34+ phenotype, and promoted proliferation and suppressed apoptosis of RAECs under hyperglycemia. Conclusion: miR145 might regulate the viability of RAECs via targeting ANGPT2 and involving NFκB signaling to exert a protective effect on diabetic vasculature. © 2020 Zhang et al.

基金：
This work was funded by projects and grants from the Scientific Research Program of Y unnan Provincial Education Bureau (2014CO12Y), the Applied Basic Research Program of the Science and T echnology Hall of Y unnan Province and Kunming Medical University (2015FB091 and 2017FE468 [−104]), and by Y unnan finan - cial social security departments (2017-1 16). Jin-Juan Zhang works at the Kunming Institute of Zoology of the Chinese Academy of Sciences, while the rest of the authors work at the First People’ s Hospital of Y unnan Province. All were engaged in the experimental and writing work of this study . Both funding and efforts are appreciated.

基金编号： −104

语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2021]版：
大类 | 3 区 医学
小类 | 4 区 内分泌学与代谢
最新[2023]版：
大类 | 3 区 医学
小类 | 4 区 内分泌学与代谢
JCR分区：
出版当年[2020]版：
Q3 ENDOCRINOLOGY & METABOLISM
最新[2023]版：
Q3 ENDOCRINOLOGY & METABOLISM

影响因子： 最新[2023版] 最新五年平均 出版当年[2020版] 出版当年五年平均 出版前一年[2019版] 出版后一年[2021版]

第一作者：
第一作者机构： [a]Clinical Medical Research Center and Yunnan Provincial Key Laboratory of Clinical Virology (2018DG010), First People’s Hospital of Yunnan Province, Affiliated Hospital of Kunming University of Science and Technology, Kunming, Yunnan 650032, China
通讯作者：
推荐引用方式(GB/T 7714)：
Zhang W,Tang X.-H,Zhang J.-J,et al.MiR145 regulates the proliferation and apoptosis of rat vascular endothelial cells under hyperglycemia by targeting the ANGPT2 gene and involving the NFκB signaling pathway(Open Access)[J].DIABETES METABOLIC SYNDROME AND OBESITY-TARGETS AND THERAPY.2020,13:4435-4446.doi:10.2147/DMSO.S273451.
APA：
Zhang, W,Tang, X.-H,Zhang, J.-J&He, Q.(2020).MiR145 regulates the proliferation and apoptosis of rat vascular endothelial cells under hyperglycemia by targeting the ANGPT2 gene and involving the NFκB signaling pathway(Open Access).DIABETES METABOLIC SYNDROME AND OBESITY-TARGETS AND THERAPY,13,
MLA：
Zhang, W,et al."MiR145 regulates the proliferation and apoptosis of rat vascular endothelial cells under hyperglycemia by targeting the ANGPT2 gene and involving the NFκB signaling pathway(Open Access)".DIABETES METABOLIC SYNDROME AND OBESITY-TARGETS AND THERAPY 13.(2020):4435-4446

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