机构：[1]Shanghai Public Health Clinical Center, Fudan University, Shanghai, 201508, China.[2]CAS Key Laboratory of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, 215163, China.[3]School of Biomedical Engineering, University of Science and Technology of China, Hefei, 260026, China.[4]Medical Laboratory of Taizhou Fourth People's Hospital, Taizhou, 225300, China.[5]Viral Hemorrhagic Fevers Research Unit, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, 200031, China.[6]Shanghai International Travel Healthcare Center, Shanghai, 200335, China.[7]Department of Dermatology and Venereology, First Affiliated Hospital of Kunming Medical University, Kunming, 650032, China.内科科室皮肤科昆明医科大学附属第一医院
Human herpesviruses are double-stranded DNA viruses that are classified into nine species. More than 90% of adults are ever infected with one or more herpesviruses. The symptoms of infection with different herpesviruses are diverse ranging from mild or asymptomatic infections to deadly diseases such as aggressive lymphomas and sarcomas. Timely and accurate detection of herpesvirus infection is critical for clinical management and treatment. In this study, we established a single-tube nonuple qPCR assay for detection of all nine herpesviruses using a 2-D multiplex qPCR method with a house-keeping gene as the internal control. The novel assay can detect and distinguish different herpesviruses with 30 to 300 copies per 25 µL single-tube reaction, and does not cross-react with 20 other human viruses, including DNA and RNA viruses. The robustness of the novel assay was evaluated using 170 clinical samples. The novel assay showed a high consistency (100%) with the single qPCR assay for HHVs detection. The features of simple, rapid, high sensitivity, specificity, and low cost make this assay a high potential to be widely used in clinical diagnosis and patient treatment.
基金：
National Science and Technology Major Project of China
(2019YFC1200603, and 2017ZX10103009-002).
第一作者机构：[1]Shanghai Public Health Clinical Center, Fudan University, Shanghai, 201508, China.[2]CAS Key Laboratory of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, 215163, China.[3]School of Biomedical Engineering, University of Science and Technology of China, Hefei, 260026, China.
通讯作者：
推荐引用方式(GB/T 7714)：
Li Yingxue,Wan Zhenzhou,Zuo Lulu,et al.A Novel 2-dimensional Multiplex qPCR Assay for Single-Tube Detection of Nine Human Herpesviruses.[J].VIROLOGICA SINICA.2021,36(4):746-754.doi:10.1007/s12250-021-00354-2.
APA：
Li Yingxue,Wan Zhenzhou,Zuo Lulu,Li Shenwei,Liu Honglian...&Zhang Chiyu.(2021).A Novel 2-dimensional Multiplex qPCR Assay for Single-Tube Detection of Nine Human Herpesviruses..VIROLOGICA SINICA,36,(4)
MLA：
Li Yingxue,et al."A Novel 2-dimensional Multiplex qPCR Assay for Single-Tube Detection of Nine Human Herpesviruses.".VIROLOGICA SINICA 36..4(2021):746-754
