机构：[1]Medical School, Kunming University of Science and Technology, Kunming, China,[2]Department of Radiology, The First Affiliated Hospital of Kunming Medical University, Kunming, China,医技科室医学影像中心放射科昆明医科大学附属第一医院[3]Department of Burn and Plastic Surgery, 926 Hospital of People’s Liberation Army, Kaiyuan, China
The protective effect of Cannabidiol on Parkinson's disease (PD) has been found in recent study. However, the specific mechanism of the protective effect of Cannabidiol on PD nerve damage require further exploration. This study aims to investigate effect of Cannabidiol on MMP-induced Neural Cells (SH-SY5Y) mitochondrial dysfunction. MMP+ and Cannabidiol were used to treat SH-SY5Y cells, the cells viability was measured by MTT assay. The expression of Tyrosine hydroxylase (TH) in cells was measured by western blotting and Immunofluorescence staining. The relationship among Cannabidiol, Silent mating type information regulation 2 homolog-1 (SIRT1) and NOTCH signaling, NF-kappa B signaling was examined by western blotting. The effect of Cannabidiol on MMP+-induced mitochondrial dysfunction of SH-SY5Y cells was measured by western blotting. Cannabidiol alleviated loss of TH expression and cytotoxicity in the MPP+-induced SH-SY5Y cells. Further mechanistic investigation showed that Cannabidiol induced SH-SY5Y cells autophagy to protects cells from mitochondrial dysfunction by upregulating SIRT1 to Inhibits NF-kappa B and NOTCH Pathways. Taken together, Cannabidiol acts as a protector in PD.
基金：
This study was supported by the National and Regional Science
Foundation Projects 81860338 and Yunnan Province Foundation
of Talent Cultivation (KKSY201960025).
第一作者机构：[2]Department of Radiology, The First Affiliated Hospital of Kunming Medical University, Kunming, China,
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
Shaolei Kang,Jinglin Li,Zhihui Yao,et al.Cannabidiol Induces Autophagy to Protects Neural Cells From Mitochondrial Dysfunction by Upregulating SIRT1 to Inhibits NF-kappa B and NOTCH Pathways[J].FRONTIERS IN CELLULAR NEUROSCIENCE.2021,15:doi:10.3389/fncel.2021.654340.
APA：
Shaolei Kang,Jinglin Li,Zhihui Yao&Jiaxin Liu.(2021).Cannabidiol Induces Autophagy to Protects Neural Cells From Mitochondrial Dysfunction by Upregulating SIRT1 to Inhibits NF-kappa B and NOTCH Pathways.FRONTIERS IN CELLULAR NEUROSCIENCE,15,
MLA：
Shaolei Kang,et al."Cannabidiol Induces Autophagy to Protects Neural Cells From Mitochondrial Dysfunction by Upregulating SIRT1 to Inhibits NF-kappa B and NOTCH Pathways".FRONTIERS IN CELLULAR NEUROSCIENCE 15.(2021)
