机构:[1]Department of Cardiology, Sichuan Academy of Medical Science &Sichuan Provincial People's Hospital, Chengdu, China四川省人民医院[2]Department of Nephrology, Sichuan Academy of Medical Science & Sichuan Provincial People's Hospital, Chengdu, China四川省人民医院[3]Department of Pulmonary and Critical Care Medicine, The First Affiliated Hospital of Kunming Medical University, Kunming, China昆明医科大学附属第一医院呼吸与危重症一科呼吸内科内科科室[4]Department of Talent Highland, First Affiliated Hospital of Xi’an Jiao Tong University, Xian, China
Myocardial infarction (MI) is the most common heart disease, and also, it is one of the leading causes of death from cardiovascular disease. It is well known that MI causes additional injury during blood flow restoration in ischaemic myocardium. Boeravinone B (BB) is a well-known antioxidant and anti-inflammatory drug. We investigated the cardioprotective effect of BB drug against isoproterenol (ISO)-induced MI in rats in this experimental study, along with we analysed its underlying mechanism. Adult Sprague Dawley (SD) rats were treated subcutaneously with ISO (45 mg/kg), then divided into groups and then given BB drug was administered orally. The cardioprotective effect of BB on ISO-induced MI rats was analysed by estimating the heart injury markers, antioxidant pro-inflammatory cytokines and inflammatory parameters. We also detected quantified expression of inflammation and apoptosis-related marker protein family. We estimated the effect of BB drug on GUT microbiota in ISO-induced MI rats and scrutinized the histopathological variations in heart tissues. BB treatment significantly (P < .001) diminished the level of heart markers such as lactate dehydrogenase (LDH), troponin (TnT), creatine kinase (CK) and creatine kinase isoenzymes MB (CK-MB). BB treatment also altered the antioxidant parameters and reduced the pro-inflammatory cytokines in the serum and tissues. Additionally, the histopathological aspects demonstrated that the pathological changes observed in the heart tissue of the ISO group rats were suppressed by the BB treatment to varying degrees. Furthermore, the expressions of caspase-3, p53, caspase-9, Bax, interleukin-6 (IL-6), cytochrome C, neutrophil gelatinase-associated lipocalin (NGAL), tumour necrosis factor-alpha (TNF-alpha), nuclear factor kappa B (NF-kappa B) and interleukin-1 beta (IL-1 beta) in the heart tissue were down-regulated whereas the Bcl-2 expression seemed to be enhanced. BB treatment not only alleviated ISO-induced gut dysbiosis by its enhanced specified Firmicutesto-Bacteroidetes (F/B) ratio but also maintained the relative abundance of major bacteria such as Clostridium IV, Butyricicoccus, Clostridium XIVs, Akkermansia and Roseburia. Collectively, our findings showed that the BB drug acted against myocardial infraction and prevented the damage by reducing the oxidative stress and controlling the inflammatory pathways, and gut microbiota.
第一作者机构:[1]Department of Cardiology, Sichuan Academy of Medical Science &Sichuan Provincial People's Hospital, Chengdu, China
共同第一作者:
通讯作者:
通讯机构:[1]Department of Cardiology, Sichuan Academy of Medical Science &Sichuan Provincial People's Hospital, Chengdu, China[*1]Department of Cardiology, Sichuan Academy of Medical Science & Sichuan Provincial People's Hospital, 610072 Chengdu, China.
推荐引用方式(GB/T 7714):
Chen Yu,Peng Lei,Shi Shaoqing,et al.Boeravinone B alleviates gut dysbiosis during myocardial infarction-induced cardiotoxicity in rats[J].JOURNAL OF CELLULAR AND MOLECULAR MEDICINE.2021,25(13):6403-6416.doi:10.1111/jcmm.16620.
APA:
Chen, Yu,Peng, Lei,Shi, Shaoqing,Guo, Gang&Wen, Heling.(2021).Boeravinone B alleviates gut dysbiosis during myocardial infarction-induced cardiotoxicity in rats.JOURNAL OF CELLULAR AND MOLECULAR MEDICINE,25,(13)
MLA:
Chen, Yu,et al."Boeravinone B alleviates gut dysbiosis during myocardial infarction-induced cardiotoxicity in rats".JOURNAL OF CELLULAR AND MOLECULAR MEDICINE 25..13(2021):6403-6416