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Umbilical Cord Mesenchymal Stem Cells Ameliorate Kidney Injury in MRL/Ipr Mice Through the TGF-β1 Pathway.

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收录情况： ◇ SCIE ◇ 预警期刊

作者：
Huang Chunkai[1,2,3] * ; Meng Mingyao[1,2,3] * ; Li Shuo[1,2,3] ; Liu Shiyuan[1,2,3] ; Li Lin[1,2,3] ; Su Yanjun[4] ; Gao Hui[1,2,3] ; He Shan[1,2,3] ; Zhao Yiyi[1,2,3] ; Zhang Min[4] ; Hou Zongliu[1,2,3] ; Wang Wenju[1,2,3] ; Wang Xiaodan[1,2,3] ;
机构： [1]Scientific Research Department, Yan’an Hospital Affiliated to Kunming Medical University, Kunming, China, [2]Key Laboratory of Tumor Immunological Prevention and Treatment of Yunnan Province, Kunming, China, [3]Yunnan Cell Biology and Clinical Translation Research Center, Kunming, China, [4]Thyroid Surgery, The First Affiliated Hospital of Kunming Medical University, Kunming, China
出处：
DOI： 10.3389/fcell.2022.876054
ISSN：

关键词： lupus nephritis umbilical cord mesenchymal stem cells podocytes TGF-β1 p-Smad3 TRAF6

摘要：
The therapeutic effects and mechanism of umbilical cord mesenchymal stem cells (UC-MSC) on kidney injury in MRL/Ipr mice were studied. UC-MSC, methylprednisolone (MP), and their combination were used to treat MRL/Ipr mice. The therapeutic effects were evaluated by renal function assessment, and HE, PAS, and Masson staining were carried out on renal tissues and visualized by electron microscopy. Subsequently, podocyte injury was detected by the presence of podocin in renal tissues by immunofluorescence. To further explore the mechanism, serum TGF-β1 was measured, and TGF-β1, p-Smad3, and TRAF6 in the renal tissue were detected by Western blotting. In vitro, TGF-β1 was used to stimulate podocytes, and the podocyte activity and changes in synaptopodin were observed after UC-MSC treatment. Significant improvements in renal function and pathological injury were observed in the UC-MSC group compared to the lupus nephritis (LN) model group. UC-MSC and MP treatment improved podocyte injury in MRL/Ipr mice. Western blot examination showed a significant increase in TGF-β1, p-Smad3, and TRAF6 expression in renal tissues of the LN model group, while significant downregulation of those proteins was observed in the UC-MSC group. After TGF-β1 stimulation in vitro, podocyte activity decreased, and UC-MSC treatment improved podocyte activity and restored synaptopodin expression. UC-MSC therapy could improve the deterioration of renal function and the pathological changes of the renal tissues in MRL/Ipr mice. Our study suggested that UC-MSC may improve kidney injury and podocyte injury in LN mice by inhibiting the TGF-β1 pathway.Copyright © 2022 Huang, Meng, Li, Liu, Li, Su, Gao, He, Zhao, Zhang, Hou, Wang and Wang.

基金：
National Natural Science Foundation of China (No. 81960136) and the Science and Technology Department of Yunnan Province (Nos. 202003AC100014 and 202101AT070243).
语种：
外文
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中科院(CAS)分区：
出版当年[2023]版：
大类 | 2 区 生物学
小类 | 2 区 发育生物学 3 区 细胞生物学
最新[2023]版：
大类 | 2 区 生物学
小类 | 2 区 发育生物学 3 区 细胞生物学
JCR分区：
出版当年[2022]版：
Q1 DEVELOPMENTAL BIOLOGY Q2 CELL BIOLOGY
最新[2023]版：
Q1 DEVELOPMENTAL BIOLOGY Q2 CELL BIOLOGY

影响因子： 最新[2023版] 最新五年平均 出版当年[2022版] 出版当年五年平均 出版前一年[2021版] 出版后一年[2023版]

第一作者：
第一作者机构： [1]Scientific Research Department, Yan’an Hospital Affiliated to Kunming Medical University, Kunming, China, [2]Key Laboratory of Tumor Immunological Prevention and Treatment of Yunnan Province, Kunming, China, [3]Yunnan Cell Biology and Clinical Translation Research Center, Kunming, China,
共同第一作者：
通讯作者：
通讯机构： [1]Scientific Research Department, Yan’an Hospital Affiliated to Kunming Medical University, Kunming, China, [2]Key Laboratory of Tumor Immunological Prevention and Treatment of Yunnan Province, Kunming, China, [3]Yunnan Cell Biology and Clinical Translation Research Center, Kunming, China,
推荐引用方式(GB/T 7714)：
Huang Chunkai,Meng Mingyao,Li Shuo,et al.Umbilical Cord Mesenchymal Stem Cells Ameliorate Kidney Injury in MRL/Ipr Mice Through the TGF-β1 Pathway.[J].FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY.2022,10:doi:10.3389/fcell.2022.876054.
APA：
Huang Chunkai,Meng Mingyao,Li Shuo,Liu Shiyuan,Li Lin...&Wang Xiaodan.(2022).Umbilical Cord Mesenchymal Stem Cells Ameliorate Kidney Injury in MRL/Ipr Mice Through the TGF-β1 Pathway..FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY,10,
MLA：
Huang Chunkai,et al."Umbilical Cord Mesenchymal Stem Cells Ameliorate Kidney Injury in MRL/Ipr Mice Through the TGF-β1 Pathway.".FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY 10.(2022)

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