Background: Macrophages can phagocytose sperm, especially damaged spermatozoa, in the female genital tract. The semenogelin I-derived peptide SgI-52 in seminal plasma exhibits seminal plasma motility inhibitor (SPMI) activity and can inhibit sperm motility. This raises the question of the role played by SPMIs in macrophage-mediated phagocytosis of sperm. We speculated that SgI-52 promotes sperm clearance by macrophages. Therefore, we investigated the phagocytosis of sperm in different states using this peptide. Methods: SgI-52 was fluorescently labeled, and its binding site for sperm was observed. The ability of macro-phages to phagocytose sperm was observed using fluorescence confocal microscopy. Spermatozoa from different sources were co-cultured with SgI-52 in BWW medium for 4 and 22 h to compare the differences in their phagocytosis by macrophages. Sperm motility, induced acrosome reaction, mitochondrial membrane potential, and ATP content were examined after incubation with SgI-52. Results: SgI-52 could bind to spermatozoa in different states, mainly to the tail, and then spread to the acrosome. This effect was more pronounced in demembranated spermatozoa. SgI-52 promoted phagocytosis of spermatozoa by macrophages, decreased the mitochondrial membrane potential, and increased the average ATP content of spermatozoa (P < 0.05). Conclusions: We found for the first time that SgI-52 can bind to spermatozoa in different states and promote their phagocytosis by macrophages. Therefore, we speculate that SgI-52 is involved in the screening of sperm in the female reproductive tract and has potential value in improving assisted reproductive technology.
基金:
Yunnan Provincial Department of Science and Technology-Kunming Medical University Joint Special Fund for Applied Foundations Key Project, China [2019FE001]; Yunnan Provincial Department of Science and Technology-Kunming Medical University Joint Special Fund for Applied Founda-tions Project, China [2014FB031]
第一作者机构:[1]Kunming Med Univ, Dept Urol, Affiliated Hosp 1, Kunming 650032, Yunnan, Peoples R China
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推荐引用方式(GB/T 7714):
He Chaoyong,Li Jiankai,Wu Zhao,et al.The semenogelin I-derived peptide SgI-52 in seminal plasma participates in sperm selection and clearance by macrophages[J].PEPTIDES.2022,153:170799.doi:10.1016/j.peptides.2022.170799.
APA:
He, Chaoyong,Li, Jiankai,Wu, Zhao,Lu, Chuncheng,Huang, Zhuo...&Zhao, Hui.(2022).The semenogelin I-derived peptide SgI-52 in seminal plasma participates in sperm selection and clearance by macrophages.PEPTIDES,153,
MLA:
He, Chaoyong,et al."The semenogelin I-derived peptide SgI-52 in seminal plasma participates in sperm selection and clearance by macrophages".PEPTIDES 153.(2022)