机构:[1]Department of Reproduction and Genetics, The First Affiliated Hospital of Kunming Medical University, Kunming, China外科科室医技科室生殖遗传科医学影像中心昆明医科大学附属第一医院
Background Humanin has reportedly been expressed in testis and spermatozoa, but no study has yet reported its presence in human seminal plasma (SP). Objective The aim of this study was to investigate the presence of humanin in human SP and to determine the correlation between humanin levels in SP/spermatozoa and sperm quality. Materials and methods Semen samples for SP/sperm humanin level measurement were collected from 164 patients who attended our andrology clinic for fertility evaluation. The localization of humanin in spermatozoa was evaluated using an immunofluorescence method, and SP/sperm humanin levels were measured with ELISA. Correlations between SP/sperm humanin levels and sperm parameters were analyzed. Results Humanin was expressed in the midpiece of the spermatozoa. Humanin concentrations in the SP ranged from 24.4 to 285.1 pg/mL, with a median of 89.7 pg/mL. The SP humanin concentrations in patients with normospermia were significantly higher than those in patients with oligospermia (p < 0.001), asthenospermia (p = 0.002), and oligoasthenospermia (p < 0.001). Spearman analysis showed a positive and significant correlation between SP humanin concentration and sperm concentration (r = 0.75, p < 0.001), and progressive sperm motility (r = 0.29, p < 0.001). Sperm humanin level was significantly and positively associated with progressive sperm motility (r = 0.70, p < 0.001). In addition, a significantly higher level of humanin was found in swim-up spermatozoa than in non-swim-up spermatozoa (p = 0.03). Conclusions Seminal plasma and sperm humanin levels were significantly and positively correlated with sperm quality, especially sperm motility. Further studies of the origin of SP humanin and its role in spermatogenesis should be conducted.
基金:
National Natural Science Foundation of ChinaNational Natural Science Foundation of China [81801416]; First Affiliated Hospital of Kunming Medical University [2017BS008]; Technology & Innovation Team of Reproduction and Genetics from Kunming Medical University [CXTD201708]
第一作者机构:[1]Department of Reproduction and Genetics, The First Affiliated Hospital of Kunming Medical University, Kunming, China
通讯作者:
通讯机构:[*1]Department of Reproduction and Genetics, The First Affiliated Hospital of Kunming Medical University, No. 295 Xi Chang Road,Kunming, 650032, China.
推荐引用方式(GB/T 7714):
Rao M.,Wu Z.,Wen Y.,et al.Humanin levels in human seminal plasma and spermatozoa are related to sperm quality[J].ANDROLOGY.2019,7(6):859-866.doi:10.1111/andr.12614.
APA:
Rao, M.,Wu, Z.,Wen, Y.,Wang, R.,Zhao, S.&Li Tang.(2019).Humanin levels in human seminal plasma and spermatozoa are related to sperm quality.ANDROLOGY,7,(6)
MLA:
Rao, M.,et al."Humanin levels in human seminal plasma and spermatozoa are related to sperm quality".ANDROLOGY 7..6(2019):859-866