机构：[1]Department of Urology, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, China四川大学华西医院[2]Biotoxin Units, Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, The Chinese Academy of Sciences, Kunming, Yunnan 650223, China[3]Department of Urology, The First Affiliated Hospital of Kunming Medical College, Kunming, Yunnan 650032, China外科科室泌尿外科昆明医科大学附属第一医院
Semenogelin I (SgI) is one of the most abundant proteins in human seminal plasma. SgI plays a key role in sperm coagulation and spermatozoon immobilization. in addition, SgI and/or its proteolytic fragments are involved in regulating spermatozoon motility, capacitation and inhibin-like activity. However, little is known about the antibacterial activity of SgI-derived peptides. By a combination of ion-exchange, gel filtration and high-performance liquid chromatography, peptides from liquefied human seminal plasma from 40 healthy donors were isolated and characterized. N-terminal amino-acid sequencing and fast atom bombardment mass spectrometry revealed that four isolated peptides were SgI-derived, namely SgI-29 (8S-113), SgI-46 (85-130), SgI-47 (85-131) and SgI-52 (85-136). Interestingly, SgI-29, SgI-46 and SgI-47 are newly identified SgI-derived peptides. Antimicrobial activity assay results indicated that synthesized SgI-29 had strong antibacterial activity toward various bacterial strains. Our results indicate that SgI can be digested into small fragments like newly identified SgI-29, SgI-46 and SgI-47 and may have diversified functions. (C) 2008 Elsevier Inc. All rights reserved.
基金：
This work was supported by the grants of ‘‘Key Research
Direction-KSCX2-YW-R-088’’ and ‘‘Western Light’’ projects
from The Chinese Academy of Sciences; and grants from
National Natural Science Foundation (30470380, 30630014,
30670412) and Yunnan Science and Technology Commission
(2005PY01-23, 2007C0006R).
第一作者机构：[1]Department of Urology, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, China[2]Biotoxin Units, Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, The Chinese Academy of Sciences, Kunming, Yunnan 650223, China[3]Department of Urology, The First Affiliated Hospital of Kunming Medical College, Kunming, Yunnan 650032, China
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
Zhao Hui,Lee Wen-Hui,Shen Ji-Hong,et al.Identification of novel semenogelin I-derived antimicrobial peptide from liquefied human seminal plasma[J].PEPTIDES.2008,29(4):505-511.doi:10.1016/j.peptides.2008.01.009.
APA：
Zhao, Hui,Lee, Wen-Hui,Shen, Ji-Hong,Li, Hong&Zhang, Yun.(2008).Identification of novel semenogelin I-derived antimicrobial peptide from liquefied human seminal plasma.PEPTIDES,29,(4)
MLA：
Zhao, Hui,et al."Identification of novel semenogelin I-derived antimicrobial peptide from liquefied human seminal plasma".PEPTIDES 29..4(2008):505-511
