详情页 - 昆明医科大学第一附属医院知识库

当前位置：首页 > 详情页

Directed osteogenic differentiation of human bone marrow mesenchymal stem cells via sustained release of BMP4 from PBVHx-based nanoparticles

31| 导出 | 链接全文 |

文献详情

资源类型：

WOS体系：

Pubmed体系：

收录情况： ◇ SCIE

作者：

机构： [1]School of Clinical Medicine, Qujing Medical College, Qujing 655000, China [2]School of Clinical Medicine, Chengdu University, Chengdu, China [3]Zigong Affiliated Hospital of Southwest Medical University, Zigong Psychiatric Research Center, Zigong Institute of Brain Science, Zigong 643002, China [4]Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, School of Medicine, Department of Life Sciences and Medicine, Northwest University, Xi'an 710069, China [5]Department of Pathology, The First Affiliated Hospital of Kunming Medical University, Kunming 650032, China

出处：

DOI：

ISSN：

关键词： BMP4 Nanoparticles Polyhydroxyalkanoates

摘要：

Bone Morphogenetic Protein 4 (BMP4) is crucial for bone and cartilage tissue regeneration, essential in medical tissue engineering, cosmetology, and aerospace. However, its cost and degradation susceptibility pose significant clinical challenges. To enhance its osteogenic activity while reducing dosage and administration frequency, we developed a novel long-acting BMP4 delivery system using poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxyhexanoate) (PBVHx) nanoparticles with soybean lecithin-modified BMP4 (sBP-NPs). These nanoparticles promote directed osteogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs) through sustained BMP4 release. sBP-NPs exhibited uniform size (100-200 nm) and surface charges, with higher BMP4 entrapment efficiency (82.63 %) compared to controls. After an initial burst release within 24 h, sBP-NPs achieved 80 % cumulative BMP4 release within 20 days, maintaining levels better than control BP-NPs with unmodified BMP4. Co-incubation and nanoparticle uptake experiments confirmed excellent biocompatibility of sBP-NPs, promoting hBMSC differentiation towards osteogenic lineage with increased expression of type I collagen, calcium deposition, and ALP activity (> 20,000 U/g protein) compared to controls. Moreover, hBMSCs treated with sBP-NPs exhibited heightened expression of osteogenic genetic markers, surpassing control groups. Hence, this innovative strategy of sustained BMP4 release from sBP-NPs holds potential to revolutionize bone regeneration in minimally invasive surgery, medical cosmetology or space environments.Copyright © 2024. Published by Elsevier B.V.

基金：

语种：

被引次数：

WOS：

PubmedID：

中科院(CAS)分区：

出版当年[2025]版：

无

最新[2023]版：

大类 | 1 区

化学

小类 | 1 区应用化学 1 区高分子科学 2 区生化与分子生物学

JCR分区：

出版当年[2024]版：

无

最新[2023]版：

Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Q1 CHEMISTRY, APPLIED Q1 POLYMER SCIENCE

影响因子： 7.7 最新[2023版] 7.7 最新五年平均 0 出版当年[2024版] 0 出版当年五年平均 7.7 出版前一年[2023版]

第一作者：

第一作者机构： [1]School of Clinical Medicine, Qujing Medical College, Qujing 655000, China [5]Department of Pathology, The First Affiliated Hospital of Kunming Medical University, Kunming 650032, China

通讯作者：

通讯机构： [1]School of Clinical Medicine, Qujing Medical College, Qujing 655000, China [2]School of Clinical Medicine, Chengdu University, Chengdu, China [3]Zigong Affiliated Hospital of Southwest Medical University, Zigong Psychiatric Research Center, Zigong Institute of Brain Science, Zigong 643002, China [4]Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, School of Medicine, Department of Life Sciences and Medicine, Northwest University, Xi'an 710069, China [*1]School of Clinical Medicine, Qujing Medical College, Qujing 655000, China.

推荐引用方式(GB/T 7714)：

APA：

MLA：

相关文献

[1]磁流体热疗对小鼠胰腺癌治疗作用的研究 [2]Remarkably enhanced thermal properties of tobacco granules with high-thermal-conductivity nanoparticles [3]Preparation of gastrodin-modified dendrimer-entrapped gold nanoparticles as a drug delivery system for cerebral ischemia-reperfusion injury [4]纳米金颗粒在头颈肿瘤诊疗中的研究进展 [5]Fe2O3纳米磁流体热疗治疗小鼠结肠癌实验研究 [6]Antibody-free detection of protein phosphorylation using intrinsic peroxidase-like activity of platinum/carbon dot hybrid nanoparticles [7]Zinc alginate hydrogels with embedded RL-QN15 peptide-loaded hollow polydopamine nanoparticles for diabetic wound healing therapy [8]Hollow polydopamine nanoparticles loading with peptide RL-QN15: a new pro-regenerative therapeutic agent for skin wounds. [9]Core-shell lipid polymer nanoparticles for combined chemo and gene therapy of childhood head and neck cancers [10]Anti-inflammatory mechanisms of neutrophil membrane-coated nanoparticles without drug loading