Hypoxia is a common phenomenon in pituitary adenomas (PAs). The role and mechanism of hypoxia in the PAs remains elusive. This work aimed to explore the effect of hypoxia on PAs in vitro. PA cells GT1-1 were cultured and treated under hypoxic condition. Cell proliferation assay showed the proliferation of PA cells was increased significantly by hypoxia treatment, with a peak at 12 hours. qPCR and western blot indicated that the expression of HIF-1 alpha, ALKBH5, and Nanog were elevated by hypoxia stimuli. In conclusion, our funding demonstrated that hypoxia could increase Nanog expression through HIF-1 alpha/ALKBH5 signaling, thereby promoting the proliferation of PA cells.
基金：
Medical Academic Leader of Yunnan Province [D201618]; Health Technology Programme of Yunnan Province [2016NS057]; Innovation Team for the Effect of Neurodevelopmental Regulatory Factors in Neural Repair and Clinical Application of Yunnan Province [2018HC084]; Youth Talent Support Program of Yunnan Province
第一作者机构：[2]Kunming Med Univ, Yunnan Key Lab Lab Med, Affiliated Hosp 1, Kunming, Yunnan, Peoples R China[3]Kunming Maternal & Child Hlth Hosp, Dept Med Genet & Prenatal Diag, Kunming, Yunnan, Peoples R China
通讯作者：
通讯机构：[1]Kunming Med Univ, Dept Neurosurg, Affiliated Hosp 1, 295 Xichang Rd, Kunming 650032, Yunnan, Peoples R China
推荐引用方式(GB/T 7714)：
Qian Yuan,Zhang Chao,Wang Wei,et al.Hypoxia promotes proliferation of pituitary adenomas by HIF-1 alpha/ALKBH5 signaling in vitro[J].INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY.2020,13(5):1030-1034.
APA：
Qian, Yuan,Zhang, Chao,Wang, Wei,Lu, Di,Li, Junjun...&Deng, Xingli.(2020).Hypoxia promotes proliferation of pituitary adenomas by HIF-1 alpha/ALKBH5 signaling in vitro.INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY,13,(5)
MLA：
Qian, Yuan,et al."Hypoxia promotes proliferation of pituitary adenomas by HIF-1 alpha/ALKBH5 signaling in vitro".INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY 13..5(2020):1030-1034
