Based on a method combining the LC-MS/MS molecular networking strategy with the conventional means of phytochemical research, the chemical constituents and the availability of Paris tengchongensis, a new species found in 2017 from Yunnan Province, were investigated for the first time. The molecular networking showed that this species contained the characteristic steroidal glycosides of the genus Paris by comparison of those of Paris polyphylla var. yunnanensis. Furthermore, the detailed investigation on the 80% EtOH extract of its rhizomes resulted to the isolation of twenty steroidal glycosides including three new spirostane-type saponins, named paristengosides A-C (1-3). Their structures were confirmed by spectroscopic analyses (HRMS and NMR) and chemical methods. The new isolates were evaluated for their cytotoxicities against two human cancer cell lines (HEL and MDA-MB-231), anti-inflammatory effects on a lipopolysaccharide (LPS)-stimulated NO production model in RAW264.7 macrophages, anti-AChE, and antimicrobial activities. The results from the molecular networking and the investigation on the chemical constituents suggested that P. tengchongensis can be used as a potential resource of Rhizoma Paridis.
基金:
National Natural Science Foundation of ChinaNational Natural Science Foundation of China [U1802287, 31770391]; Innovation Team of the Ministry of Education [IRT-17R49]; Major Biomedical Project of Yunnan Province [2018ZF005, 2019ZF010]; Ten Thousand Talents Plan of Yunnan Province -Program for Industrial Technology Leading Talents; State Key Laboratory of Phytochemistry and Plant Resources in West China [P2018-KF11, P2019-ZZ02]; State Key Laboratory of Natural and Biomimetic Drugs [K20180207]; open research project of "Cross-Cooperative Team" of the Germplasm Bank of Wild Species, Kunming Institute of Botany, CAS; Foundation for Distinguished Young Talents in Higher Education of Anhui Province [2013SQRL057ZD]
第一作者机构:[1]Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China[2]Chinese Acad Sci, Kunming Inst Bot, Yunnan Key Lab Nat Med Chem, Kunming 650201, Yunnan, Peoples R China[5]Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式(GB/T 7714):
Wang Jie,Li Dan,Ni Wei,et al.Molecular networking uncovers steroidal saponins of Paris tengchongensis[J].FITOTERAPIA.2020,145:doi:10.1016/j.fitote.2020.104629.
APA:
Wang, Jie,Li, Dan,Ni, Wei,Qin, Xu-Jie,Liu, Hui...&Liu, Hai-Yang.(2020).Molecular networking uncovers steroidal saponins of Paris tengchongensis.FITOTERAPIA,145,
MLA:
Wang, Jie,et al."Molecular networking uncovers steroidal saponins of Paris tengchongensis".FITOTERAPIA 145.(2020)