机构：[1]State Key Laboratory of Phytochemistry and Plant Resources in West China, Yunnan Key Laboratory of Natural Medicinal Chemistry, Kunming Institute of Botany,Chinese Academy of Sciences, Kunming, 650201, China[2]University of Chinese Academy of Sciences, Beijing, 100039, China[3]Key Laboratory of Medicinal Chemistry for Natural Resource of Ministry of Education and Yunnan Province, School of Chemical Science and Technology, Yunnan University, Kunming, 650091, China[4]Department of Dermatology, The First Affiliated Hospital of Kunming Medical University, Kunming, 650032, China内科科室皮肤科昆明医科大学附属第一医院[5]Kunming Botanee Bio-Technique Co. Ltd, Kunming, 650106, China
Nine undescribed polyacetylated 18-norspirostanol saponins, trilliumosides A-J, were obtained after a guidance based on a molecular networking strategy from the rhizomes of Trillium tschonoskii. Their structures were established by analysis of comprehensive spectroscopic data and chemical methods after their isolation in pure form. All isolated saponins were evaluated for their cytotoxicities against five selected human cancer cell lines (Huh7,A549,MCF-7,HepG2, and MOLT-4) and anti-inflammatory effects on a lipopolysaccharide (LPS) stimulated NO production model in RAW264.7 macrophages. Trilliumoside D showed significant cytotoxicity against MOLT-4 cell lines with an IC50 value of 1.0 +/- 0.1 mu M, whereas trilliumosides H and I displayed remarkable anti-inflammatory effects on NO production with inhibitory rates of 56.3 +/- 1.5 and 56.2 +/- 2.246 at the concentration of 1.0 mu M, respectively.
基金：
National Natural Science Foundation of China of ChinaNational Natural Science Foundation of China [U1802287, 31600283, 31570363, 31970377]; Innovation Team of the Ministry of Education [IRT-17R49]; Youth Innovation Promotion Association CAS [2019381]; Key Research and Development Plan of Yunnan Province-Special Project of Science and Technology in Yunnan Province [2017IB007]; Major Biomedical Project of Yunnan Province [2017AB001, 2018ZF005]; Guiding Program of Interdisciplinary Studies from Kunming Institute of Botany, CAS [KIB2017004]
第一作者机构：[1]State Key Laboratory of Phytochemistry and Plant Resources in West China, Yunnan Key Laboratory of Natural Medicinal Chemistry, Kunming Institute of Botany,Chinese Academy of Sciences, Kunming, 650201, China[2]University of Chinese Academy of Sciences, Beijing, 100039, China
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
Li Dan,Liu Hui,Ni Wei,et al.Molecular networking-based strategy for the discovery of polyacetylated 18-norspirostanol saponins from Trillium tschonoskii maxim[J].PHYTOCHEMISTRY.2019,168:doi:10.1016/j.phytochem.2019.112125.
APA：
Li, Dan,Liu, Hui,Ni, Wei,Xiao, Wei-Lie,He, Li...&Liu, Hai-Yang.(2019).Molecular networking-based strategy for the discovery of polyacetylated 18-norspirostanol saponins from Trillium tschonoskii maxim.PHYTOCHEMISTRY,168,
MLA：
Li, Dan,et al."Molecular networking-based strategy for the discovery of polyacetylated 18-norspirostanol saponins from Trillium tschonoskii maxim".PHYTOCHEMISTRY 168.(2019)
