机构：[1]Yunnan Key laboratory for Basic Research on Bone and Joint Diseases, Kunming University, Kunming 650214, China.[2]Second Affiliated Hospital of Kunming Medical University, Kunming 650500, China.[3]Department of Emergency Surgery, The First People’s Hospital of Yunnan Province, Affiliated Hospital of Kunming University of Science and Technology, Kunming 650032, China.云南省第一人民医院[4]Bone and Joint Rehabilitation Department, Disabled Rehabilitation Centre of Yunnan Province, Kunming 650032, China.[5]First Affiliated Hospital of Kunming Medical University, Kunming 650031, China昆明医科大学附属第一医院
BackgroundBone damage is a condition that affects the quality of life of patients. Mesenchymal stem cells (MSCs) are important for bone repair. Osteoking is a natural compound in traditional Chinese Medicine used to treat bone diseases; however, the effect of Osteoking on the differentiation of MSCs has not been reported. In this study, we aimed to investigate the effect of Osteoking on the osteogenic and adipogenic differentiation potential of rat bone marrow mesenchymal stem cells (rbMSCs).MethodsThe effects of Osteoking on the proliferation and differentiation of rbMSCs were investigated. Different concentrations of Osteoking were prepared, and its cytotoxicity was evaluated by CCK-8 assay. The expression of osteogenic and adipogenic genes were determined, and several staining methods were used to reveal the osteogenic and adipogenic differentiation potential of rbMSCs.ResultsOur results show that appropriate concentrations of Osteoking can enhance osteogenic differentiation of rbMSCs and reduce adipogenic differentiation without any effect on proliferation. This may be related to the changes in related gene expression.ConclusionOsteoking enhances osteogenic differentiation and inhibits adipogenic differentiation of rbMSCs. Therefore, Osteoking may have a therapeutic potential for treating bone disease caused by changes in differentiation function of MSCs.
基金：
Yunnan Province Project Education Fund [ZD2012006]; Yunnan Local Colleges Applied Basic Research Projects [2017FH001-098]
第一作者机构：[1]Yunnan Key laboratory for Basic Research on Bone and Joint Diseases, Kunming University, Kunming 650214, China.
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推荐引用方式(GB/T 7714)：
Yu Congtao,Dai Lifen,Ma Zhaoxia,et al.Effect of Osteoking on the osteogenic and adipogenic differentiation potential of rat bone marrow mesenchymal stem cells in vitro[J].BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE.2019,19:doi:10.1186/s12906-019-2435-6.
APA：
Yu, Congtao,Dai, Lifen,Ma, Zhaoxia,Zhao, Hongbin,Yuan, Yong...&Hu, Min.(2019).Effect of Osteoking on the osteogenic and adipogenic differentiation potential of rat bone marrow mesenchymal stem cells in vitro.BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE,19,
MLA：
Yu, Congtao,et al."Effect of Osteoking on the osteogenic and adipogenic differentiation potential of rat bone marrow mesenchymal stem cells in vitro".BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE 19.(2019)
