机构：[1]Yunnan Key Laboratory for Basic Research on Bone and Joint Diseases and Yunnan Stem Cell TranslationalResearch Center, Kunming University, Kunming, Yunnan 650214[2]Department of Nuclear Medicine, The First AffiliatedHospital of Kunming Medical University, Kunming, Yunnan 650032核医学科内科科室[3]Department of Orthopaedics, The Second AffiliatedHospital of Kunming Medical University, Kunming, Yunnan 650101外科科室骨科昆明医科大学附属第一医院[4]Department of Orthopaedics, First People's Hospital ofYunnan Province, Kunming, Yunnan 650011云南省第一人民医院[5]Department of Pharmacy, Kunming Medical University, Kunming, Yunnan 650500云南省第一人民医院[6]Center of Tree Shrew Germplasm Resources, Institute of Medical Biology, Chinese Academy ofMedical Sciences and Peking Union Medical College, Kunming, Yunnan 650118, P.R. China
Osteoporosis (OP) treatment has always been challenging for elderly menopausal females. An animal model with a closer genetic association to human OP is essential for treatment research. Given its close genetic association to primates, the tree shrew is a suitable candidate for meeting the requirements for such an animal model. In the present study, a tree shrew OP model induced by ovariectomy (OVX), was established. Evaluation by multiple analysis methods, including blood biochemical indicators, uterus coefficients, micro-computed tomography analysis, histochemical analysis and scanning electron microscopic observation indicated that OVX was an appropriate method to establish the OP model in tree shrews. In addition, the biomolecular characteristics of OVX-induced osteoporosis were also assessed by transcriptome sequencing and bioinformatics analysis. The present study provides the methods used to confirm the successful establishment of the OP model in tree shrew, and suggests that the OP model is appropriate for human OP research.
基金：
The present study was supported by the National Science and Key Technology Support Program (grant no. 2014BAI01B01-07).
第一作者机构：[1]Yunnan Key Laboratory for Basic Research on Bone and Joint Diseases and Yunnan Stem Cell TranslationalResearch Center, Kunming University, Kunming, Yunnan 650214
共同第一作者：
通讯作者：
通讯机构：[2]Department of Nuclear Medicine, The First AffiliatedHospital of Kunming Medical University, Kunming, Yunnan 650032[*1]Yunnan Key Laboratory for Basic Research on Bone and Joint Diseases and Yunnan Stem Cell Translational Research Center, Kunming University, 2 Puxin Road, Kunming, Yunnan 650214, P.R. China[*2]Center of Tree Shrew Germplasm Resources, Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, 935 Jiaoling Road, Kunming, Yunnan 650118, P.R. China
推荐引用方式(GB/T 7714)：
YAOLONG WANG,ZHAOXIA MA,YUANYUAN ZHENG,et al.Establishment of an osteoporosis model in tree shrews by bilateral ovariectomy and comprehensive evaluation[J].EXPERIMENTAL AND THERAPEUTIC MEDICINE.2019,17(5):3644-3654.doi:10.3892/etm.2019.7339.
APA：
YAOLONG WANG,ZHAOXIA MA,YUANYUAN ZHENG,BAOLING LIU,PENGFEI BAO...&MIN HU.(2019).Establishment of an osteoporosis model in tree shrews by bilateral ovariectomy and comprehensive evaluation.EXPERIMENTAL AND THERAPEUTIC MEDICINE,17,(5)
MLA：
YAOLONG WANG,et al."Establishment of an osteoporosis model in tree shrews by bilateral ovariectomy and comprehensive evaluation".EXPERIMENTAL AND THERAPEUTIC MEDICINE 17..5(2019):3644-3654
