机构：[1]Institute of Neuroscience, Kunming Medical University, Kunming, Yunnan 650500[2]Kunming Lin Ming Biological Technology Co., Ltd., Kunming, Yunnan 650000[3]Department of Physical Education, Hainan Normal University, Haikou, Hainan 571100[4]Department of Ultrasonic Cardiogram, Kunming Children's Hospital,Kunming Medical University, Kunming, Yunnan 650228[5]Departments of Pharmacy,First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650000医技科室药剂科昆明医科大学附属第一医院[6]Departments of Emergency,First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650000内科科室急诊医学科昆明医科大学附属第一医院[7]Institute of Neuroscience, and Animal Zoology Department, Kunming Medical University, Kunming, Yunnan 650500, P.R. China
Tree shrews are most closely related to the primates and so possess a number of advantages in experimental studies; they have been used as an animal model in bacterial and virus infection, cancer, endocrine system disease, and certain nervous system diseases. Their olfactory ensheathing cells (OECs) are able to release several cytokines to promote neuronal survival, regeneration and remyelination. The present study used western blot analysis to identify antibody specificity in protein extracts from whole tree shrew brains to identify the specificity of p75 nerve growth factor receptor (NGFR) derived from rabbits (75 kDa). OECs were cultured and isolated, then stained and identified using the antibodies for p75NGFR. To investigate the capacity of OECs to express cytokines and growth factors, microarray technology was used, and the analysis revealed that OECs were able to express 9,821 genes. Of these genes, 44 genes were from the neurotrophic factor family, which may indicate their potential in transplantation in vivo. The present study considered the function of OECs as revealed by other studies, and may contribute to future research.
基金：
Chinese Ministry of the National Science-Technology Support Plan Project [2014BAI01B01]
第一作者机构：[1]Institute of Neuroscience, Kunming Medical University, Kunming, Yunnan 650500[2]Kunming Lin Ming Biological Technology Co., Ltd., Kunming, Yunnan 650000
共同第一作者：
通讯作者：
通讯机构：[6]Departments of Emergency,First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650000[*1]Institute of Neuroscience, Kunming Medical University, 191 Western Renmin Road, Kunming, Yunnan 650500, P.R. China[*2]Department of Emergency, First Affiliated Hospital of Kunming Medical University, 295 Xichang Road, Kunming, Yunnan 650000, P.R. China
推荐引用方式(GB/T 7714)：
Lin Na,Dong Xiu-Juan,Wang Ting-Yong,et al.Characteristics of olfactory ensheathing cells and microarray analysis in Tupaia belangeri (Wagner, 1841)[J].MOLECULAR MEDICINE REPORTS.2019,20(2):1819-1825.doi:10.3892/mmr.2019.10422.
APA：
Lin, Na,Dong, Xiu-Juan,Wang, Ting-Yong,He, Wen-Ji,Wei, Jing...&Wang, Ting-Hua.(2019).Characteristics of olfactory ensheathing cells and microarray analysis in Tupaia belangeri (Wagner, 1841).MOLECULAR MEDICINE REPORTS,20,(2)
MLA：
Lin, Na,et al."Characteristics of olfactory ensheathing cells and microarray analysis in Tupaia belangeri (Wagner, 1841)".MOLECULAR MEDICINE REPORTS 20..2(2019):1819-1825
