机构：[1]Research Institute of Surgery, Daping Hospital ,Third Military Medical University, Chongqing 400042, China[2]State Key Laboratory of Trauma, Burn and Combined Injury, Chongqing 400042, China[3]Department of minimal invasive neurosurgery of the first affiliated hospital, Kunming Medical University, Kunming, 650032, China外科科室神经外科神经外一科（神经外科）昆明医科大学附属第一医院[4]Physiological department, Chongqing Medical University,Chongqing 400042, China
In recent years, olfactory ensheathing cells (OECs) have been used as a therapeutic strategy to repair the anatomical structure and promote the function recovery of injured spinal cord in both animal and human. In this study, OECs were transplanted into contused spinal cords of adult rats. After dorsal laminectomy at T10 vertebra, spinal cord was injured by a force of 10 g with NYU II impactor from 25 mm above the exposed cord. The contused spinal cord received injections of OECs in DMEM or DMEM alone at one week after injury. The migration and distribution of OECs in the contused spinal cord were observed by the light microscope. The intact tissue area, injured tissue area, cavity size, number of myelinated nerve fibers and neurons labeled by CB-HRP in T8 segment were measured and counted by the semi-quantitative techniques at 6 weeks after transplantation. Locomotor ability and conductive function of the spinal cord were evaluated by the BBB score and cortical somatosensory evoked potentials (CSEP) recording. OECs were found in both lesion site and tissue near the lesion. The intact tissue area was significantly larger in the OECs-transplanted rats than that in the DMEM-injected animals, whereas the injured tissue area was significantly smaller in the OECs-rats than that in the DMEM-rats. The number of myelinated nerve fibers in the lesion site and preserved neurons in 18 was significantly greater in the OECs-group than in the DMEM-group, but the cavity size detected was not significantly different between the two groups. The BBB score and CSEP recording showed a better performance of locomotor ability and conductive function in the OECs-transplanted rats than in the DMEM-injected animals. These results indicate that OECs can counteract secondary tissue degeneration after spinal cord injury. Although they cannot reduce the cavity formation, they can promote morphological preservation and functional improvement of the contused spinal cord. (C) 2011 Elsevier B.V. All rights reserved.
基金：
State Key Laboratory of Trauma, Burn and Combined Injury [SKLZZ200803]; Special Funds for Major State Basic Research Project, China [2005CB522604]
第一作者机构：[1]Research Institute of Surgery, Daping Hospital ,Third Military Medical University, Chongqing 400042, China[2]State Key Laboratory of Trauma, Burn and Combined Injury, Chongqing 400042, China[*1]Research Institute of Surgery, Daping Hospital, Third Military Medical University, Changjiang Zhilu 10, Daping, Chongqing 400042, China
通讯作者：
通讯机构：[*1]Research Institute of Surgery, Daping Hospital, Third Military Medical University, Changjiang Zhilu 10, Daping, Chongqing 400042, China
推荐引用方式(GB/T 7714)：
Li Bing Cang,Li Yue,Chen Li Fa,et al.Olfactory ensheathing cells can reduce the tissue loss but not the cavity formation in contused spinal cord of rats[J].JOURNAL OF THE NEUROLOGICAL SCIENCES.2011,303(1-2):67-74.doi:10.1016/j.jns.2011.01.013.
APA：
Li, Bing Cang,Li, Yue,Chen, Li Fa,Chang, Jie Yuan&Duan, Zhao Xiao.(2011).Olfactory ensheathing cells can reduce the tissue loss but not the cavity formation in contused spinal cord of rats.JOURNAL OF THE NEUROLOGICAL SCIENCES,303,(1-2)
MLA：
Li, Bing Cang,et al."Olfactory ensheathing cells can reduce the tissue loss but not the cavity formation in contused spinal cord of rats".JOURNAL OF THE NEUROLOGICAL SCIENCES 303..1-2(2011):67-74
