Involvement of Akt/CREB signaling pathways in the protective effect of EPA against interleukin-1 beta-induced cytotoxicity and BDNF down-regulation in cultured rat hippocampal neurons
机构：[1]School of Medicine, Yunnan University, 2 Cuihu Bei Road, Kunming 650091, Yunnan, People’s Republic of China[2]The First Afliated Hospital of Kunming Medical University, 295 Xichang Road, Kunming 650031, Yunnan, People’s Republic of China昆明医科大学附属第一医院
Background: Our published data have indicated that the omega-3 polyunsaturated fatty acid eicosapentaenoic acid (EPA) provides beneficial effects by attenuating neuronal damage induced by interleukin-1 beta(IL-1 beta), and up-regulation of the expression of brain-derived neurotrophic factor (BDNF) represents a crucial part in the neuroprotective effect of EPA. However, the mechanisms of how EPA regulates BDNF expression remains incompletely understood. The present study investigated the role of Akt/CREB signaling in the effect of EPA on BDNF expression and its neuroprotective effect. Results: The present results showed that IL-1 beta reduced hippocampal neuronal viability and that EPA showed a concentration-dependent neuroprotective effect, but the neuroprotective effects of EPA were abolished by inhibition of Akt using KRX-0401, an inhibitor of Akt. Treatment of hippocampal neurons with EPA also ameliorated the decrease in Akt and CREB phosphorylation induced by IL-1 beta and BDNF down-regulation mediated by IL-1 beta. However, inhibition of Akt reversed the effect of EPA on levels of p-Akt, p-CREB, and BDNF. Conclusions: Our data indicate that EPA elicited neuroprotection toward IL-1 beta-induced cell damage and BDNF decrease and that its effects potentially occurred via the Akt/CREB signaling pathway.
基金：
National Nature Science Foundation of ChinaNational Natural Science Foundation of China [81360179, 81560197]; Science and Technology project of Yunnan Provincial Science and Technology Department [2016FB132, 2015FB118]
第一作者机构：[1]School of Medicine, Yunnan University, 2 Cuihu Bei Road, Kunming 650091, Yunnan, People’s Republic of China
通讯作者：
推荐引用方式(GB/T 7714)：
Dong YiLong,Pu KangJing,Duan WenJing,et al.Involvement of Akt/CREB signaling pathways in the protective effect of EPA against interleukin-1 beta-induced cytotoxicity and BDNF down-regulation in cultured rat hippocampal neurons[J].BMC NEUROSCIENCE.2018,19:doi:10.1186/s12868-018-0455-7.
APA：
Dong, YiLong,Pu, KangJing,Duan, WenJing,Chen, HuiCheng,Chen, LiXing&Wang, YanMei.(2018).Involvement of Akt/CREB signaling pathways in the protective effect of EPA against interleukin-1 beta-induced cytotoxicity and BDNF down-regulation in cultured rat hippocampal neurons.BMC NEUROSCIENCE,19,
MLA：
Dong, YiLong,et al."Involvement of Akt/CREB signaling pathways in the protective effect of EPA against interleukin-1 beta-induced cytotoxicity and BDNF down-regulation in cultured rat hippocampal neurons".BMC NEUROSCIENCE 19.(2018)
