机构:[1]Department of Gastrointestinal Surgery, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650031外科科室普通外科普通外一科(胃肠与疝外科)昆明医科大学附属第一医院[2]Department of Anesthesiology, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650031外科科室麻醉手术科(医技)昆明医科大学附属第一医院[3]Department of Hepatobiliary Surgery, The Affiliated Ganmei Hospital of Kunming Medical University, Kunming, Yunnan 650011, P.R. China
The aim of the present study was to investigate the effects of Toll-like receptor (TLR) 9 and CpG oligodeoxynucleotide (CpG-ODN)1826 on sodium taurocholate-induced acute pancreatitis (AP) rats at different time points. Pathological examination indicated that the severity of pancreatic tissue damage following AP increased with time. Additionally, TLR9 protein levels were upregulated after AP, and were higher at 6 h compared with at 3 h. Subsequently, the TLR9 protein levels were downregulated at 12 h, but remained higher than the control group. In rats subjected to AP, tumor necrosis factor (TNF- protein expression levels and serum amylase (AMS) in the serum were increased until 12 h. The expression level of TNF- protein in the AP 12 h group was higher than that in the AP 3 and 6 h groups. In addition, following CpG-ODN1826 administration, the morphology of pancreatic tissue appeared worse compared with that in the AP only groups. Furthermore, CpG-ODN1826 administration induced an increase in TLR9 expression levels compared with the AP alone group at 0, 3, 6 and 12 h. TNF- in the CpG + AP 12 h group was upregulated compared with that in the CpG + AP 3 and 6 h groups; however, no change was observed between 3 and 6 h. Thus, these data indicate that CpG-ODN1826 aggravates sodium taurocholate-induced pancreas damage in rats.
基金:
Yunnan Provincial Science and Technology Department Foundation of China [2015FB024]
第一作者机构:[1]Department of Gastrointestinal Surgery, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650031
共同第一作者:
通讯作者:
通讯机构:[*1]Department of Gastrointestinal Surgery, The First Affiliated Hospital of Kunming Medical University, 295 Xi Chang Road, Kunming, Yunnan 650031, P.R. China
推荐引用方式(GB/T 7714):
Sun Liang,Li Yuanhua,Luo Huayou,et al.Effects of Toll-like receptor 9 and CpG oligodeoxynucleotides 1826 on sodium taurocholate-induced acute pancreatitis rats[J].MOLECULAR MEDICINE REPORTS.2018,18(4):3818-3824.doi:10.3892/mmr.2018.9346.
APA:
Sun, Liang,Li, Yuanhua,Luo, Huayou,Wang, Kunhua,Li, Li&Zeng, Yujian.(2018).Effects of Toll-like receptor 9 and CpG oligodeoxynucleotides 1826 on sodium taurocholate-induced acute pancreatitis rats.MOLECULAR MEDICINE REPORTS,18,(4)
MLA:
Sun, Liang,et al."Effects of Toll-like receptor 9 and CpG oligodeoxynucleotides 1826 on sodium taurocholate-induced acute pancreatitis rats".MOLECULAR MEDICINE REPORTS 18..4(2018):3818-3824