机构：[1]Technology Transfer Center, Kunming Medical University, Kunming, China[2]Department of Neurology, the First Affiliated Hospital, Kunming Medical University, Kunming, China内科科室神经内科昆明医科大学附属第一医院[3]Department of Anatomy, Kunming Medical University, Kunming, China[4]Department of Physiology, Kunming Medical University, Kunming, China[5]Functional Experimental Center, Kunming Medical University, Kunming, China[6]Kunming Medical UniversityThe first affiliated hospital of Kunming Medical university, Kunming, China昆明医科大学附属第一医院医学检验科医技科室
BackgroundKeloid is a common abnormal cutaneous fibroproliferative disorder. However, the process underlying keloid pathogenesis still remains to be unclear. ObjectiveTo integrated analyse the miRNA expression profiles of keloid. MethodsWe performed miRNA expression profiles analysis of 3 paired keloid and normal tissue samples by miRNA microarray. Differentially expressed miRNAs were further performed integrative analysed and validated using quantitative reverse transcription polymerase chain reaction (qRT-PCR). After predicting target genes, we constructed the miRNA-target genes interaction network and carried out bioinformatics analysis. ResultsThe results revealed that 264 miRNAs were significantly altered. The expression of high frequency miRNAs which included miRNA-199a-5p, miRNA-21-5p, miRNA-214-5p, miRNA-424-5p, and miRNA-205-5p was further evaluated. The gene ontology (GO) analyses and the top enriched Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways showed these target genes were associated with MAPK signaling pathway and HIF-1 signaling pathway. ConclusionThis study revealed the profiling of miRNAs in keloid that are potentially implicated in the development of this disease and could serve as novel diagnostic and therapeutic target of keloid.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China [81560502]; National Natural Science Foundation of Yunnan Province [2017FB116]; Education Department Fund of Yunnan Province [2015Z082]; Hundred-Talent Program of Kunming Medical University
第一作者机构：[1]Technology Transfer Center, Kunming Medical University, Kunming, China[2]Department of Neurology, the First Affiliated Hospital, Kunming Medical University, Kunming, China
共同第一作者：
通讯作者：
通讯机构：[*1]Technology Transfer Center, Kunming Medical University, 1168 West Chunrong Road, Yuhua Avenue, Chenggong District, Kunming, Yunnan, 650500, China
推荐引用方式(GB/T 7714)：
Zhong Lianmei,Bian Ligong,Lyu Jing,et al.Identification and integrated analysis of microRNA expression profiles in keloid[J].JOURNAL OF COSMETIC DERMATOLOGY.2018,17(5):917-924.doi:10.1111/jocd.12706.
APA：
Zhong, Lianmei,Bian, Ligong,Lyu, Jing,Jin, Huiyan,Liu, Zijie...&Lu, Di.(2018).Identification and integrated analysis of microRNA expression profiles in keloid.JOURNAL OF COSMETIC DERMATOLOGY,17,(5)
MLA：
Zhong, Lianmei,et al."Identification and integrated analysis of microRNA expression profiles in keloid".JOURNAL OF COSMETIC DERMATOLOGY 17..5(2018):917-924
