机构：[1]Department of Surgical Oncology, The First Affiliated Hospital of Kunming Medical University, Kunming 650032, China昆明医科大学附属第一医院肿瘤科外科科室[2]Department of Vascular Surgery, The First Affiliated Hospital of Kunming Medical University, Kunming 650032, China外科科室普通外科普通外三科(血管外科)昆明医科大学附属第一医院[3]Department of imaging, The First Affiliated Hospital of Kunming Medical University, Kunming 650032, China医技科室医学影像中心昆明医科大学附属第一医院[4]Department of Hepatology, The First Affiliated Hospital of Kunming Medical University, Kunming 650032, China昆明医科大学附属第一医院器官移植中心外科科室[5]Department of Pathology, The First Affiliated Hospital of Kunming Medical University, Kunming 650032, China医技科室病理科昆明医科大学附属第一医院
Background: LncRNA NKILA has been reported to play a role as tumor suppressor in the development of various human cancers. However, functionality of NKILA in the development of colon cancer still hasn't been reported. Methods: In this study, expression of NKILA in tumor tissues of patients with different stages of colon cancer was detected by qRT-PCR. Effects of NKILA knockdown and overexpression on migration and invasion of different colorectal adenocarcinoma cell lines were investigated by Transwell invasion and migration assay, respectively. Interaction between NKILA and PI3K/Akt pathway was explored by western blot. Results: We found that expression level of NKILA was reduced with the development of colon cancer. NKILA knockdown promoted the migration and invasion of different colorectal adenocarcinoma cell lines, induced the activation of PI3K/Akt pathway and promoted tumor growth in mice, while NKILA overexpression played an opposite role. Conclusions: We conclude that NKILA can suppress proliferation and migration of colon cancer cell by inhibiting the activation of PI3K/Akt pathway.
基金：
Basic Application Fund of Yunnan Province [2015FB039]
第一作者机构：[1]Department of Surgical Oncology, The First Affiliated Hospital of Kunming Medical University, Kunming 650032, China
共同第一作者：
通讯作者：
通讯机构：[*1]Department of Surgical Oncology, The First Affiliated Hospital of Kunming Medical University, No. 295 Xichang Road, Kunming 650032, China
推荐引用方式(GB/T 7714)：
Huang Jian,Zhao Lingfeng,Chen Wei,et al.LncRNA NKILA suppresses colon cancer cell proliferation and migration by inactivating PI3K/Akt pathway[J].TRANSLATIONAL CANCER RESEARCH.2018,7(6):1431-1438.doi:10.21037/tcr.2018.11.05.
APA：
Huang, Jian,Zhao, Lingfeng,Chen, Wei,Duan, Jian,Shrestha, Dibesh...&Zhu, Zhu.(2018).LncRNA NKILA suppresses colon cancer cell proliferation and migration by inactivating PI3K/Akt pathway.TRANSLATIONAL CANCER RESEARCH,7,(6)
MLA：
Huang, Jian,et al."LncRNA NKILA suppresses colon cancer cell proliferation and migration by inactivating PI3K/Akt pathway".TRANSLATIONAL CANCER RESEARCH 7..6(2018):1431-1438
