机构：[1]Department of Neurology, Chongqing Key Laboratory of Neurology, The First Affiliated Hospital of Chongqing Medical University, 1 You Yi Road, Chongqing 400016, China内科系统神经内科(ICU)重庆医科大学附属第一医院[2]Department of Neurology, The First Affiliated Hospital of Kunming Medical University, Kunming 650032, China内科科室神经内科昆明医科大学附属第一医院[3]Department of Neurology, The First Affiliated Hospital of Xinxiang Medical University, Weihui 453100, Henan Province, China
Repulsive guidance molecule a (RGMa) is a membrane-bound protein that inhibits axon outgrowth in the central nervous system. Temporal lobe epilepsy (TLE) is a common neurological disorder characterized by recurrent spontaneous seizures. To explore the role of RGMa in epilepsy, we investigated the expression of RGMa in patients with TLE, pilocarpine-induced rat model, and pentylenetetrazol kindling model of epilepsy, and then we performed behavioral, histological, and electrophysiological analysis by lentivirus-mediated overexpression of RGMa in the hippocampus of animal model. We found that RGMa was significantly decreased in TLE patients and in experimental rats from 6 h to 60 days after pilocarpine-induced seizures. In two types of epileptic animal models, pilocarpine-induced model and pentylenetetrazol kindling model, overexpression of RGMa in the hippocampus of rats exerted seizure-suppressant effects. The reduced spontaneous seizures were accompanied by attenuation of hippocampal mossy fiber sprouting. In addition, overexpression of RGMa inhibited hyperexcitability of hippocampal neurons via suppressing NMDAR-mediated currents in Mg2+-free-induced organotypic slice model. Collectively, these results demonstrate that overexpression of RGMa could be an alternative strategy for epilepsy therapy.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China [81071039]
第一作者机构：[1]Department of Neurology, Chongqing Key Laboratory of Neurology, The First Affiliated Hospital of Chongqing Medical University, 1 You Yi Road, Chongqing 400016, China[2]Department of Neurology, The First Affiliated Hospital of Kunming Medical University, Kunming 650032, China
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
Chen Ling,Gao Baobing,Fang Min,et al.Lentiviral Vector-Induced Overexpression of RGMa in the Hippocampus Suppresses Seizures and Mossy Fiber Sprouting[J].MOLECULAR NEUROBIOLOGY.2017,54(2):1379-1391.doi:10.1007/s12035-016-9744-2.
APA：
Chen, Ling,Gao, Baobing,Fang, Min,Li, Jie,Mi, Xiujuan...&Wang, Xuefeng.(2017).Lentiviral Vector-Induced Overexpression of RGMa in the Hippocampus Suppresses Seizures and Mossy Fiber Sprouting.MOLECULAR NEUROBIOLOGY,54,(2)
MLA：
Chen, Ling,et al."Lentiviral Vector-Induced Overexpression of RGMa in the Hippocampus Suppresses Seizures and Mossy Fiber Sprouting".MOLECULAR NEUROBIOLOGY 54..2(2017):1379-1391
