Background: Cardiac resynchronization therapy (CRT) is efficacious in the treatment of chronic heart failure (CHF); however, because it is non-physiological, some patients are unresponsive. The present study used rate-adaptive atrioventricular delay (RAAVD) to track the physiological atrioventricular delay and investigated the effects of left univentricular pacing on CRT. Material/Methods: Patients with CHF fulfilling the indication of CRT Class I were categorized into a left univentricular pacing by RAAVD group and a standard biventricular pacing group. Preoperative and postoperative electrocardiography QRS duration, echocardiographic indicators, quality of life, cardiac function, and annual treatment cost were estimated. The standard deviation (R-S/R-SD5) of the S/R ratio in lead V-1 at 5 heart rate segments in the left univentricular pacing by RAAVD was calculated, and the accuracy of RAAVD in tracking the physiological AV delay was evaluated. Results: The comparison between the left univentricular pacing by RAAVD group and the standard biventricular pacing group after operation showed a significantly reduced QRS duration (137 +/- 11 vs. 144 +/- 11 ms, P<0.05), increased AVVTI (21.84 +/- 2.25 vs. 20.45 +/- 2.12 cm, P<0.05), reduced IVMD (64.27 +/- 12.29 vs. 71.39 +/- 13.64 ms, P<0.05), decreased MRA (3.09 +/- 1.12 vs. 3.73 +/- 1.19 cm(2), P<0.05), and reduced average annual treatment cost (1.30 +/- 0.1 vs. 2.20 +/- 0.2 million Yuan, P<0.05). The R-S/R-SD5 in the left univentricular pacing by RAAVD group was negatively correlated with improvements in cardiac function (r=-0.394, P= 0.031). Conclusions: Left univentricular pacing by RAAVD has treatment effects similar to those of standard biventricular pacing, and is an economically and physiologically effective method for biventricular systolic resynchronization in the treatment of CHF.