机构：[1]Department of Dermatology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325035[2]Department of Dermatology, The First Affiliated Hospital of Kunming Medical University,Yunnan Provincial Institute of Dermatology, Kunming, Yunnan 650032内科科室皮肤科昆明医科大学附属第一医院[3]Department of Dermatology, The First Affiliated Hospital of Fujian Medical University,Fuzhou, Fujian 350005, P.R. China
The present study examined the mechanisms of high mobility group box 1 (HMGB1)-induced cell migration in human cutaneous squamous cell carcinoma (CSCC) SCC13 cells. Western blotting, a chemotaxis assay and ELISA were performed to analyze HMGB1 level in SCC13 cells and its ability to regulate tumor metastasis. The results demonstrated a significantly higher level of HMGB1 in the SCC13 cell supernatant compared with the human epidermoid carcinoma A431 cell supernatant. Administration of HMGB1 to the SCC13 cells caused cell migration, which occurred in a time- and dose-dependent manner. Moreover, HMGB1 significantly activated the phosphosphoinositide 3-kinase (PI3K)/AKT and mitogen-activated protein kinase (MAPK) signaling pathways by an increased level of phosphorylation in p85PI3K, AKT, p38 and p42/44 MAPK. Taken together, these data suggest that HMGB1 regulates tumor metastasis in CSCC via the PI3K/AKT and MAPK signaling pathways.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China [30860257, 81101188, 810701297]
第一作者机构：[1]Department of Dermatology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325035[*1]Department of Dermatology, The First Affiliated Hospital of Wenzhou Medical University, 3 Fuxue Road, Wenzhou, Zhejiang 325035, P.R. China
通讯作者：
通讯机构：[*1]Department of Dermatology, The First Affiliated Hospital of Wenzhou Medical University, 3 Fuxue Road, Wenzhou, Zhejiang 325035, P.R. China[*2]Department of Dermatology, The First Affiliated Hospital of Fujian Medical University, 20 Cha Zhong Road, Fuzhou, Fujian 350005, P.R. China
推荐引用方式(GB/T 7714)：
Sun Ying,Tu Ying,He Li,et al.High mobility group box 1 regulates tumor metastasis in cutaneous squamous cell carcinoma via the PI3K/AKT and MAPK signaling pathways[J].ONCOLOGY LETTERS.2016,11(1):59-62.doi:10.3892/ol.2015.3843.
APA：
Sun, Ying,Tu, Ying,He, Li,Ji, Chao&Cheng, Bo.(2016).High mobility group box 1 regulates tumor metastasis in cutaneous squamous cell carcinoma via the PI3K/AKT and MAPK signaling pathways.ONCOLOGY LETTERS,11,(1)
MLA：
Sun, Ying,et al."High mobility group box 1 regulates tumor metastasis in cutaneous squamous cell carcinoma via the PI3K/AKT and MAPK signaling pathways".ONCOLOGY LETTERS 11..1(2016):59-62
