HIV genotyping has led to conflicting results between laboratories. Therefore, identifying the most accurate gene combinations to sequence remains a priority. Datasets of Chinese HIV subtypes based on several markers and deposited in PubMed, Metstr, CNKI, and VIP databases between 2000 and 2015 were studied. In total, 9177 cases of amplification-positive samples from 26 provinces of China were collected and used to classify HIV subtypes based on eight individual genes or a combination thereof. CRF01_ AE, CRF07_ BC, CRF08_ BC and B were the prevalent HIV subtypes in China, accounting for 84.07% of all genotypes. Gag/Env sequencing classified a greater number of HIV subtypes compared to other genes or combination of gene fragments. The geographical distribution of Gag and Gag/Env genotypes was similar to that observed with all genetic markers. Further principal component analysis showed a significantly different geographical distribution pattern of HIV in China for HIV genotypes detected with Gag/Env, which was in line with the distribution of all HIV genotypes in China. Gag/Env sequences had the highest diversity of the eight markers studied, followed by Gag and Gag/Pol/Env; Pol/Env polymorphisms were the least divergent. Gag/Env can serve as a high-resolution marker for HIV genotyping.
基金:
Foundation for Science and Technology Planning Project of Yunnan Provincial Bureau of Health [2012WS63]; Key Joint Funds of the Natural Science Foundation of Yunnan Province; Kunming Medical University [2014FB021]; Yunnan Institute of Digestive Disease Institute [2014NS121]; National Science Foundation of ChinaNational Natural Science Foundation of China [81360069]; Academician Workstation of Yunnan Province; Foundation for Innovative Group of the Gastrointestinal Surgery of Yunnan Province [2012HC013]; Foundation of Medical Leading Talent of Yunnan Province [L-201205]
第一作者机构:[1]Kunming Univ Sci & Technol, Affiliated Hosp, Kunming, Yunnan Province, Peoples R China[2]First Peoples Hosp Yunnan Prov, Kunming, Yunnan Province, Peoples R China[4]Kunming Univ Sci & Technol, Fac Med, Kunming, Yunnan Province, Peoples R China
共同第一作者:
通讯作者:
通讯机构:[1]Kunming Univ Sci & Technol, Affiliated Hosp, Kunming, Yunnan Province, Peoples R China[3]Kunming Med Univ, Affiliated Hosp 1, Yunnan Inst Digest Dis, Kunming, Yunnan Province, Peoples R China
推荐引用方式(GB/T 7714):
Ren L.,Wang H. W.,Xu Y.,et al.Sequencing of Gag/Env association with HIV genotyping resolution and HIV-related epidemiologic studies of HIV in China[J].GENETICS AND MOLECULAR RESEARCH.2016,15(4):-.doi:10.4238/gmr15048870.
APA:
Ren, L.,Wang, H. W.,Xu, Y.,Feng, Y.,Zhang, H. F.&Wang, K. H..(2016).Sequencing of Gag/Env association with HIV genotyping resolution and HIV-related epidemiologic studies of HIV in China.GENETICS AND MOLECULAR RESEARCH,15,(4)
MLA:
Ren, L.,et al."Sequencing of Gag/Env association with HIV genotyping resolution and HIV-related epidemiologic studies of HIV in China".GENETICS AND MOLECULAR RESEARCH 15..4(2016):-