机构：[1]Laboratory of Molecular Cardiology, Department of Cardiology, The First Affiliated Hospital of Kunming Medical College, 295 Xichang Road, Kunming, Yunnan 650032, China内科科室心脏内科昆明医科大学附属第一医院云南省第一人民医院[2]Laboratory of Structural Biology, Tsinghua University, Beijing 100084, China[3]Laboratory for Conservation and Utilization of Bio-resources, Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan University, Kunming 650091, China[4]Tianjin State Laboratory of Protein Science, College of Life Sciences, Nankai University, Tianjin 300071, China
The gene SSO0495 (proC), which encodes pyrroline-5-carboxylate reductase (P5CR) from the thermoacidophilic archeon Sulfolobus solfataricus P2 (Ss-P5CR), was cloned and expressed. The purified recombinant enzyme catalyzes the thioproline dehydrogenase with concomitant oxidation of NAD(P)H to NAD(P)(+). This archeal enzyme has an optimal alkaline pH in this reversible reaction and is thermostable with a half-life of approximately 30 min at 80 degrees C. At pH 9.0, the reverse activation rate is nearly 3-fold higher than at pH 7.0. The homopolymer was characterized by cross-linking and size exclusion gel filtration chromatography. Ss-P5CR was crystallized by the hanging-drop vapor-diffusion method at 37 degrees C. Diffraction data were obtained to a resolution of 3.5 angstrom and were suitable for X-ray structure determination. (C) 2008 Elsevier Inc. All rights reserved.
基金：
Chinese Academy of Sciences, Yunnan ProvinceChinese Academy of Sciences [2006C0071M, 2007C0001R]; First Affiliated Hospital of Kunming Medical College [2006bs08]; NSFCNational Natural Science Foundation of China [30860278]
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2010]版：
大类|4 区生物
小类|4 区生化研究方法4 区生化与分子生物学4 区生物工程与应用微生物
最新[2023]版：
大类|4 区生物学
小类|4 区生化研究方法4 区生化与分子生物学4 区生物工程与应用微生物
JCR分区：
出版当年[2009]版：
Q3BIOTECHNOLOGY & APPLIED MICROBIOLOGYQ3BIOCHEMISTRY & MOLECULAR BIOLOGYQ4BIOCHEMICAL RESEARCH METHODS
最新[2023]版：
Q4BIOCHEMICAL RESEARCH METHODSQ4BIOCHEMISTRY & MOLECULAR BIOLOGYQ4BIOTECHNOLOGY & APPLIED MICROBIOLOGY
第一作者机构：[1]Laboratory of Molecular Cardiology, Department of Cardiology, The First Affiliated Hospital of Kunming Medical College, 295 Xichang Road, Kunming, Yunnan 650032, China[*1]Laboratory of Molecular Cardiology, Department of Cardiology, The First Affiliated Hospital of Kunming Medical College, 295 Xichang Road, Kunming, Yunnan 650032, China
通讯作者：
通讯机构：[*1]Laboratory of Molecular Cardiology, Department of Cardiology, The First Affiliated Hospital of Kunming Medical College, 295 Xichang Road, Kunming, Yunnan 650032, China
推荐引用方式(GB/T 7714)：
Meng Zhaohui,Liu Zhe,Lou Zhiyong,et al.Purification, characterization and crystallization of pyrroline-5-carboxylate reductase from the hyperthermophilic archeon Sulfolobus Solfataricus[J].PROTEIN EXPRESSION AND PURIFICATION.2009,64(2):125-130.doi:10.1016/j.pep.2008.10.018.
APA：
Meng, Zhaohui,Liu, Zhe,Lou, Zhiyong,Gong, Xiaocui,Cao, Yi...&Rao, Zihe.(2009).Purification, characterization and crystallization of pyrroline-5-carboxylate reductase from the hyperthermophilic archeon Sulfolobus Solfataricus.PROTEIN EXPRESSION AND PURIFICATION,64,(2)
MLA：
Meng, Zhaohui,et al."Purification, characterization and crystallization of pyrroline-5-carboxylate reductase from the hyperthermophilic archeon Sulfolobus Solfataricus".PROTEIN EXPRESSION AND PURIFICATION 64..2(2009):125-130
