机构：[1]Department of Animal Toxinology, Kunming Institute of Zoology, The Chinese Academy of Sciences, 32 East Jiao Chang Road, Kunming, Yunnan 650223, China[2]The First Affiliated Hospital of Kunming Medical College, Kunming, Yunnan 650032, China昆明医科大学附属第一医院云南省第一人民医院
Amphibian skin is a rich resource of bioactive peptides like proline-rich bombesin from frog Bombina maxima. A novel cDNA clone encoding a precursor protein that comprises proline-rich bombesin and a novel peptide, designated as bombestatin, was isolated from a skin cDNA library of B. maxima. The predicted primary structure of the novel peptide is WEVLLNVALIRLELLSCRSSKDQDQKESCGMHSW, in which two cysteines form a disulfide bond. A BLAST search of databases did not detect sequences with significant similarity. Bombestatin possesses dose-dependent contractile activity on rat stomach strips. The differences between cDNAs encoding PR-bombesin plus bombestatin and PR-bombesin alone are due to fragment insertions located in 3'-coding region and 3'-untranslational region, respectively. (c) 2005 Elsevier B.V. All rights reserved.
基金：
This work was supported by ‘‘Western Light Project’’ from
The Chinese Academy of Sciences to Dr. W-H. Lee and Dr. Y.
Zhang; and grants from National Natural Science Foundation
(30170195, 30470380) and Yunnan Science and Technology
Commission (2003C0066M).
第一作者机构：[1]Department of Animal Toxinology, Kunming Institute of Zoology, The Chinese Academy of Sciences, 32 East Jiao Chang Road, Kunming, Yunnan 650223, China[2]The First Affiliated Hospital of Kunming Medical College, Kunming, Yunnan 650032, China
通讯作者：
推荐引用方式(GB/T 7714)：
Ji-Hong Shen,Shu-Bai Liu,Ying-Xia Zhang,et al.Cloning of novel bombesin precursor cDNAs from skin of Bombina maxima[J].REGULATORY PEPTIDES.2005,132(1-3):102-106.doi:10.1016/j.regpep.2005.09.007.
APA：
Ji-Hong Shen,Shu-Bai Liu,Ying-Xia Zhang,Yang Jin,Wen-Hui Lee&Yun Zhang.(2005).Cloning of novel bombesin precursor cDNAs from skin of Bombina maxima.REGULATORY PEPTIDES,132,(1-3)
MLA：
Ji-Hong Shen,et al."Cloning of novel bombesin precursor cDNAs from skin of Bombina maxima".REGULATORY PEPTIDES 132..1-3(2005):102-106
