机构：[1]Department of Animal Toxinology, Kunming Institute of Zoology, The Chinese Academy of Sciences, 32 East Jiao Chang Road, Kunming 650223, Yunnan, China[2]Graduate School of The Chinese Academy of Sciences, Beijing 100039, China[3]The First Affiliated Hospital of Kunming Medical College, Kunming, Yunnan 650032, China昆明医科大学附属第一医院泌尿外一科泌尿外科外科科室
Amphibian skin contains rich neuropeptides. In the present study, a novel neuromedin U (NmU) analog was isolated from skin secretions of Chinese red belly Load Bombina maxima. Being 17-amino acids long, its primary structure was established as DSSGIVGRPFFLFRPRN-NH2, in which the C-terminal 8-residue segment (FFLFRPRN) is the same as that of rat NmU, while the N-terminal part DSSGIVGRP shows a great sequence variation compared with those of NmU peptides from different resources. The peptide, named Bm-NmU-17, was found to elicit concentration-dependent contractile effects on smooth muscle of rat uterus horns. The cDNA Structure of the peptide, as obtained by a 3'-RACE strategy and subsequently cloning from a skin cDNA library, was found to contain a coding region of 438 nucleotides. The encoded precursor is composed of 145 amino acids with a single copy of Bm-NmU-17 located towards the C-terminus. The sequence of the peptide is preceded by a dibasic site (Lys-Arg) and followed by the sequence of Gly-Arg-Lys, providing the sites of cleavage and releasing of the mature peptide. (c) 2005 Elsevier B.V. All rights reserved.
基金：
This work was supported by the grants of bWestern Light
ProjectQ and bTenth Five Years PlanQ pre-research project
from The Chinese Academy of Sciences; and grants from
National Natural Science Foundation (30170195, 30200044,
30470380) and Yunnan Science and Technology Commission (2001C0061M, 2001C0062M and 2003C0066M)
第一作者机构：[1]Department of Animal Toxinology, Kunming Institute of Zoology, The Chinese Academy of Sciences, 32 East Jiao Chang Road, Kunming 650223, Yunnan, China
通讯作者：
推荐引用方式(GB/T 7714)：
Lee WH,Liu SB,Shen JH,et al.Identification and molecular cloning of a novel neuromedin U analog from the skin secretions of toad Bombina maxima[J].REGULATORY PEPTIDES.2005,129(1-3):43-47.doi:10.1016/j.regpep.2005.01.007.
APA：
Lee, WH,Liu, SB,Shen, JH,Jin, Y,Lai, R&Zhang, Y.(2005).Identification and molecular cloning of a novel neuromedin U analog from the skin secretions of toad Bombina maxima.REGULATORY PEPTIDES,129,(1-3)
MLA：
Lee, WH,et al."Identification and molecular cloning of a novel neuromedin U analog from the skin secretions of toad Bombina maxima".REGULATORY PEPTIDES 129..1-3(2005):43-47
